Exposure to cetyl pyridinium chloride and loss of integrity of cell wall of mycobacteria.

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dc.contributor.authorSekar, Gomathi
dc.contributor.authorVanaja, Kumar
dc.contributor.authorGomathiand, N S
dc.contributor.authorSelvakumar, N
dc.date.accessioned2015-07-17T11:48:11Z
dc.date.available2015-07-17T11:48:11Z
dc.date.issued2013-10
dc.description.abstractBackground: Cetyl pyridinium chloride (CPC) liquefied sputum was shown to reduce AFB smear positivity presumably damaging cell wall of M. tuberculosis. Settings: National Institute for Research in Tuberculosis, Chennai, (Tamil Nadu). Objective: To assess the cell wall damage of mycobacteria in CPC liquefied sputum, by Transmission Electron Microscopy (TEM) and mycobacteriophage adsorption studies. Methods: Pooled sputum sample from smear positive pulmonary TB patients was homogenized and liquefied with CPC. It was examined in TEM daily for four days, to assess cell wall damage of M. tuberculosis, and photomicrographs were taken. M. smegmatis mc2155, treated with CPC, was infected with mycobacteriophage (phAE129) to study phage adsorption on cell wall and plaque formation. CPC untreated sputum and M. smegmatis formed controls. Results: Photomicrographs showed that cell wall of M. tuberculosis was intact in controls and damaged in CPC preserved sputum for 96 hours. Plaque formation was seen and absent respectively in CPC untreated and treated M. smegmatis cells. Conclusion: Exposure to CPC damaged the cell wall of M. tuberculosis within 96 hours. Mycobacteriophage failed to form plaques after M. smegmatis mc2155 was treated with CPC implying inhibition of phage adsorption on damaged cell wall and thus providing a clue for poor staining and smear positivity in microscopy.en_US
dc.identifier.citationSekar Gomathi, Vanaja Kumar, Gomathiand N S, Selvakumar N. Exposure to cetyl pyridinium chloride and loss of integrity of cell wall of mycobacteria. Indian Journal of Tuberculosis. 2013 Oct; 60(4): 223-226.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/159975
dc.language.isoenen_US
dc.source.urihttps://medind.nic.in/ibr/t13/i4/ibrt13i4p223.pdfen_US
dc.subjectM. tuberculosisen_US
dc.subjectCetyl pyridinium chlorideen_US
dc.subjectCell wallen_US
dc.subjectMycobacteriophagesen_US
dc.subjectTEMen_US
dc.subject.meshCell Wall --chemistry
dc.subject.meshCell Wall --physiology
dc.subject.meshCetylpyridinium --physiology
dc.subject.meshMicroscopy, Electron, Transmission --methods
dc.subject.meshMycobacteriophages --cytology
dc.subject.meshMycobacteriophages --physiology
dc.subject.meshMycobacterium tuberculosis --cytology
dc.subject.meshMycobacterium tuberculosis --physiology
dc.titleExposure to cetyl pyridinium chloride and loss of integrity of cell wall of mycobacteria.en_US
dc.typeArticleen_US
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