Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application.

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dc.contributor.authorPongsunk, Sen_US
dc.contributor.authorEkpo, Pen_US
dc.contributor.authorDharakul, Ten_US
dc.date.accessioned2009-05-27T17:09:49Z
dc.date.available2009-05-27T17:09:49Z
dc.date.issued1996-06-01en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractHybridomas secreting monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei were produced by immunizing BALB/cJ mice with crude culture filtrate of B. pseudomallei. Two monoclonal antibodies were found to be highly specific to B.pseudomallei as tested by indirect enzyme-linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from B. pseudomallei, B. cepacia, Pseudomonas aeruginosa, P.putida, and Escherichia coli. One of the specific MAbs, clone SP-M, IgM subclass, could directly agglutinate all 42 B. pseudomallei, isolates. The study has shown that the agglutinating MAb has potential for rapid identification of B. pseudomallei in primary bacterial culture from clinical specimens. The antibody can be used in bacteriology laboratories to reduce time of biochemical methods, which require a few days.en_US
dc.description.affiliationDepartment of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationPongsunk S, Ekpo P, Dharakul T. Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application. Asian Pacific Journal of Allergy and Immunology. 1996 Jun; 14(1): 43-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36589
dc.language.isoengen_US
dc.subject.meshAgglutinationen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal --diagnostic useen_US
dc.subject.meshBurkholderia pseudomallei --immunologyen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshMiceen_US
dc.subject.meshRabbitsen_US
dc.titleProduction of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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