Diagnosis of hepatitis C virus infection by ELISA, RIBA and RT-PCR: a comparative evaluation.

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dc.contributor.authorMohan, K Ven_US
dc.contributor.authorMurugavel, K Gen_US
dc.contributor.authorRajanikanth,en_US
dc.contributor.authorMathews, Sen_US
dc.contributor.authorRaghuram, Ken_US
dc.contributor.authorRajasambandam, Pen_US
dc.contributor.authorMurali, Aen_US
dc.contributor.authorSrinivas, Uen_US
dc.contributor.authorMathiazhagan,en_US
dc.contributor.authorPalaniswamy, K Ren_US
dc.contributor.authorPanda, S Ken_US
dc.contributor.authorThyagarajan, S Pen_US
dc.date.accessioned1999-04-13en_US
dc.date.accessioned2009-05-29T03:07:35Z
dc.date.available1999-04-13en_US
dc.date.available2009-05-29T03:07:35Z
dc.date.issued1999-04-13en_US
dc.description.abstractOBJECTIVES: To evaluate the efficacy of second-generation ELISA (ELISA-2), third-generation ELISA (ELISA-3) and third-generation recombinant immunoblot assay (RIBA 3.0) for detection of antibodies to hepatitis C virus (anti-HCV) in comparison with reverse transcriptase-polymerase chain reaction (RT-PCR) to detect HCV RNA for the diagnosis of hepatitis C. METHODS: Sera of 108 patients with chronic liver disease (CLD) were analyzed by ELISA-2, ELISA-3, RIBA 3.0 and RT-PCR in the first part of the study; in the second part, sera of 105 patients with non-chronic liver disease were evaluated with ELISA-3, RIBA 3.0 and RT-PCR. RESULTS: In the CLD group, anti-HCV was positive in 4.6%, 14.8% and 16.6% by ELISA-2, ELISA-3 and RIBA 3.0, respectively. Among these anti-HCV positive cases, HCV RNA was positive in 100%, 58.9% and 64%, respectively. ELISA-2 did not give false-positive results, but missed substantial number of anti-HCV positive cases (p < 0.001). In the second group, anti-HCV was positive in 76.3% by ELISA-3 and 68.6% by RIBA 3.0 (p:ns). HCV-RNA was positive in 88.7% of ELISA- and RIBA-positive cases; in 60% of ELISA-positive, RIBA-indeterminate cases; and in 46.4% of ELISA-negative, RIBA-negative cases. CONCLUSIONS: ELISA-2 is not a suitable assay for routine screening. ELISA-3 was at par with RIBA 3.0 and it can be recommended for routine screening for anti-HCV. RT-PCR for HCV is of value in detecting early viremic, anti-HCV negative cases; this may be of importance in the treatment of hepatitis C.en_US
dc.description.affiliationDepartment of Microbiology, Dr ALMPGIBMS, Taramani, Chennai.en_US
dc.identifier.citationMohan KV, Murugavel KG, Rajanikanth , Mathews S, Raghuram K, Rajasambandam P, Murali A, Srinivas U, Mathiazhagan , Palaniswamy KR, Panda SK, Thyagarajan SP. Diagnosis of hepatitis C virus infection by ELISA, RIBA and RT-PCR: a comparative evaluation. Indian Journal of Gastroenterology. 1999 Apr-Jun; 18(2): 73-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/64965
dc.language.isoengen_US
dc.source.urihttps://www.indianjgastro.comen_US
dc.subject.meshAdulten_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshHepacivirus --isolation & purificationen_US
dc.subject.meshHepatitis C --diagnosisen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshMaleen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleDiagnosis of hepatitis C virus infection by ELISA, RIBA and RT-PCR: a comparative evaluation.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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