Estimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin.

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dc.contributor.authorMichalska, Marta
dc.contributor.authorKozakiewicz, Marcin
dc.contributor.authorBodek, Andrzej
dc.contributor.authorBodek, Kazimiera Henryka
dc.date.accessioned2015-06-23T09:30:37Z
dc.date.available2015-06-23T09:30:37Z
dc.date.issued2015-04
dc.description.abstractThe effect of homogeneous fibrin (Fb), collagen (Coll) and composite fibrin-heparin (Fb-Hp), fibrin-collagen (Fb-Coll) membranes on in vitro release of platelet-derived growth factor (PDGF-BB) was evaluated in the presence or absence of amoxicillin using of the ELISA immunoassay test. Amoxicillin concentration was determined spectrophotometrically at 272 nm. The process of the PDGF-BB growth factor and amoxicillin release from the studied membranes was of a two-phase nature in the majority of the systems analysed. The PDGF-BB was released in the highest amount from the Coll membrane (M7) without the presence of amoxicillin – 546.2 ±7.47 pg, t0.5 = 0.88 h and 202.5 ± 6.83 pg, t0.5 = 26.65 h during the first phase and second phase, respectively. The lowest PDGF-BB release was observed from composite M4 (Fb-Hp) membrane – 5.88 ± 0.81 pg, t0.5 = 1.69 h; and 110.2 ± 6.48 pg, t0.5 = 855.6 h during first and second phase respectively. An optimal release of amoxicillin was observed in the case of the composite M6 (Fb-Coll) membrane – only in the second phase: 64.2 ± 7.8 mg, t0.5 = 83.5 h. The lowest and delayed amoxicillin release was achieved for M4 membrane (approx. 17.1 ± 1.12 mg, t0.5 = 46.5 h). The results of the PDGF-BB release and amoxicillin from membranes indicated a correlation between the level of release and composition of the film. Our results suggested that fibrin and collagen membranes may be beneficial to enhance periodontal bone regeneration.en_US
dc.identifier.citationMichalska Marta, Kozakiewicz Marcin, Bodek Andrzej, Bodek Kazimiera Henryka. Estimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin. Indian Journal of Biochemistry & Biophysics. 2015 Apr; 52 (2): 196-202.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158221
dc.language.isoenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/31531en_US
dc.subjectPDGF-BB releaseen_US
dc.subjectAmoxicillinen_US
dc.subjectFibrin-collagen membranesen_US
dc.subject.meshAmoxicillin --analysis
dc.subject.meshAmoxicillin --chemistry
dc.subject.meshCollagen --analysis
dc.subject.meshCollagen --chemistry
dc.subject.meshEnzyme-Linked Immunosorbent Assay
dc.subject.meshFibrin --analysis
dc.subject.meshFibrin --chemistry
dc.subject.meshPlatelet-Derived Growth Factor --chemistry
dc.subject.meshProto-Oncogene Proteins c-sis --chemistry
dc.titleEstimation of the use of fibrin and collagen membranes as carriers for platelet-derived growth factor-BB (PDGF-BB) in the presence of amoxicillin.en_US
dc.typeArticleen_US
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