Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant

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dc.contributor.authorMahant, Sen_US
dc.contributor.authorSharma, AKen_US
dc.contributor.authorGehlot, Ven_US
dc.contributor.authorMukhopadhyay, AKen_US
dc.contributor.authorChhawchharia, Aen_US
dc.contributor.authorDutta, Sen_US
dc.contributor.authorAgarwal, Aen_US
dc.contributor.authorSom, Aen_US
dc.contributor.authorDas, Ken_US
dc.contributor.authorDas, Ren_US
dc.date.accessioned2020-04-23T07:38:38Z
dc.date.available2020-04-23T07:38:38Z
dc.date.issued2019-09
dc.description.abstractPurpose: Helicobacter pylori causes various gastro-intestinal diseases. Antibiotic resistance to commonly used antibiotics for the treatment of H. pylori infection is the major cause for treatment failure. The aim of this study is to determine the antimicrobial susceptibility pattern for clarithromycin and levofloxacin and find the evolutionary relationship of the partial sequence of 23S rRNA and gyraseA gene of H. pylori by phylogenetic analysis. Materials and Methods: A total of 46 H. pylori strains were tested for clarithromycin and levofloxacin susceptibility pattern and phylogenetic tree were reconstructed by PhyML software. Results: In this study, we observed that only 6.5% of North-East Indian H. pylori strains were resistant for clarithromycin showing mutation at A2143G and T2182C positions of 23S rRNA gene. Resistance for levofloxacin was observed in 89.1% of the H. pylori strains showing mutations at asparagine to lysine at 87 and aspartic acid to glycine/tyrosine/asparagine at 91 positions of gyraseA gene. The phylogenetic tree of the partial sequence of 23S rRNA and gyraseA gene depicts that the North-East Indian strains falls in different cluster when compared to other countries. Conclusions: Resistance for clarithromycin was less in North-East Indian strains but high for levofloxacin indicating that first-line therapy may be best and effective for eradication of H. pylori in this region. This study is the first report that showed antibiotic susceptibility pattern for clarithromycin and levofloxacin by mutation analysis. By partial sequencing of 23s rRNA and gyraseA gene, we found that North-East Indian strains are geographically distinct.en_US
dc.identifier.affiliationsAmity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsCentre of Bioinformatics, University of Allahabad, Allahabad, Uttar Pradesh, Indiaen_US
dc.identifier.affiliationsDivision of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, Indiaen_US
dc.identifier.affiliationsDepartment of Gastroenterology, Gauhati Medical College, Guwahati, Assam, Indiaen_US
dc.identifier.affiliationsDepartment of Gastroenterology and Hepatology, Max Super Specialty Hospital, Vaishali, Ghaziabad, Uttar Pradesh, Indiaen_US
dc.identifier.citationMahant S, Sharma AK, Gehlot V, Mukhopadhyay AK, Chhawchharia A, Dutta S, Agarwal A, Som A, Das K, Das R. Geographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistant. Indian Journal of Medical Microbiology. 2019 Sep; 37(3): 337-344en_US
dc.identifier.issn0255-0857
dc.identifier.issn1998-3646
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/198910
dc.languageenen_US
dc.publisherIndian Association of Medical Microbiologistsen_US
dc.relation.issuenumber3en_US
dc.relation.volume37en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmm.IJMM_19_158en_US
dc.subjectClarithromycinen_US
dc.subjectHelicobacter pylorien_US
dc.subjectlevofloxacinen_US
dc.subjectphylogenyen_US
dc.subjectresistanceen_US
dc.titleGeographically distinct North-East Indian Helicobacter pylori strains are highly sensitive to clarithromycin but are levofloxacin resistanten_US
dc.typeJournal Articleen_US
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