Electrophoretic patterns of human rotavirus strains prevailing among hospitalized children with acute gastroenteritis.

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dc.contributor.authorPaul, S Ken_US
dc.contributor.authorAhmed, M Uen_US
dc.contributor.authorTabassum, Sen_US
dc.contributor.authorAlam, M Men_US
dc.contributor.authorIslam, M Nen_US
dc.date.accessioned2006-01-10en_US
dc.date.accessioned2009-05-27T03:53:13Z
dc.date.available2006-01-10en_US
dc.date.available2009-05-27T03:53:13Z
dc.date.issued2006-01-10en_US
dc.descriptionMymensingh Medical Journal.en_US
dc.description.abstractPolyacrylamide gel electrophoresis (PAGE) and silver staining was applied to detect rotavirus dsRNA from acute diarrheic stool of 365 hospitalized children below 5 years of age. The study was conducted at Mymensingh and Sunamgonj districts, Bangladesh from January 2002 to February 2003. Among 345 stool specimens tested, 86 (24.9%) were positive by PAGE. The rate of infection was highest in 0-12 months of age and declined significantly with increasing age. Males were slightly more infected than females and infection rate was more in winter. Twelve different electropherotypes were identified, of them eight were long and four were short. RNA profiles of the analyzed specimens, 88.6% were long and 11.4% were short patterns. Two of these long patterns (2F N 1 L, 2F N 3(U) L) circulated through out the study period and a single type was predominant (2F N 1 L). Mixed electropherotypes were also detected. Electropherotyping technique can be applied routinely to study the prevalence and epidemiological features of rotavirus infection. It is an excellent method for studying genomic variation, tracing mixed infections, detecting atypical rotaviruses lacking group-antigen and characterizing virus strains in outbreaks.en_US
dc.description.affiliationDepartment of Microbiology, Mymensingh Medical College.en_US
dc.identifier.citationPaul SK, Ahmed MU, Tabassum S, Alam MM, Islam MN. Electrophoretic patterns of human rotavirus strains prevailing among hospitalized children with acute gastroenteritis. Mymensingh Medical Journal. 2006 Jan; 15(1): 49-54en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/1322
dc.language.isoengen_US
dc.subject.meshAcute Diseaseen_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshDiarrhea --diagnosisen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFemaleen_US
dc.subject.meshGastroenteritis --diagnosisen_US
dc.subject.meshHospitalizationen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshMaleen_US
dc.subject.meshRotavirus --isolation & purificationen_US
dc.titleElectrophoretic patterns of human rotavirus strains prevailing among hospitalized children with acute gastroenteritis.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
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