The administration of peroxisome proliferator-activated receptors α/γ agonist TZD18 inhibits cell growth and induces apoptosis in human gastric cancer cell lines

Simple item page
dc.contributor.authorMa, Yuhongen_US
dc.contributor.authorWang, Binen_US
dc.contributor.authorLi, Lingen_US
dc.contributor.authorWang, Fangen_US
dc.contributor.authorXia, Xichaoen_US
dc.date.accessioned2020-11-18T10:07:29Z
dc.date.available2020-11-18T10:07:29Z
dc.date.issued2019-01
dc.description.abstractAim of Study: This study is to investigate the effects of a novel peroxisome proliferator-activated receptor (PPAR) α/γ dual agonist TZD18 on cell growth, apoptosis, caspase activity, mitochondrial membrane potential, cytochrome c release, and apoptotic-related protein expression in MKN-45 cells. Materials and Methods: 3-(4, 5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide assay against various human cancer cell lines was performed to investigate the whether TZD18 could in reduce the proliferation rates of cancer cells. The percentages of apoptotic cells and mitochondrial membrane potential level were determined by flow cytometry. The subcellular localization of cytochrome c was examined by immunofluorescence microscopy. Western blotting assay was performed to reveal the expression of apoptosis-related proteins. Results: The results showed that the administration of TZD18 could inhibit the growth of MKN-45 cells in a dose- and time-dependent manner. In addition, the apoptotic ratio increased sharply along with a significant increase of caspase activities, mitochondrial membrane potential, and cytochrome c release following TZD18 exposure. The expression of Bax and p27kip1 increased significantly, whereas the expression level of Bcl-2 protein was downregulated. Conclusion: These results indicated that the administration of PPAR α/γ agonist TZD18 may inhibit cell growth by inducing the apoptotic process in MKN-45 cellsen_US
dc.identifier.affiliationsDepartment of Basic Medicine Courses, Nanyang Medical College, Nanyang 473003, PR Chinaen_US
dc.identifier.citationMa Yuhong, Wang Bin, Li Ling, Wang Fang, Xia Xichao. The administration of peroxisome proliferator-activated receptors α/γ agonist TZD18 inhibits cell growth and induces apoptosis in human gastric cancer cell lines. Journal of Cancer Research and Therapeutics. 2019 Jan; 15(1): 120-125en_US
dc.identifier.issn0973-1482
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/213511
dc.languageenen_US
dc.publisherWolters Kluwer India Pvt. Ltd.en_US
dc.relation.issuenumber1en_US
dc.relation.volume15en_US
dc.source.urihttps://dx.doi.org//10.4103/0973-1482.208753en_US
dc.subjectApoptosisen_US
dc.subjectgastric cancer cellen_US
dc.subjectMKN-45 cellsen_US
dc.subjectperoxisome proliferator-activated receptors α/γ dual agonisten_US
dc.titleThe administration of peroxisome proliferator-activated receptors α/γ agonist TZD18 inhibits cell growth and induces apoptosis in human gastric cancer cell linesen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijcrt2019v15n1p120.pdf
Size:
1.32 MB
Format:
Adobe Portable Document Format
Download
Collections
Journal of Cancer Research and Therapeutics