Channeling of the intermediates and catalytic facilitation to Rubisco in a multienzyme complex of Calvin cycle enzymes.

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dc.contributor.authorSainis, J Ken_US
dc.contributor.authorJawali, Nen_US
dc.date.accessioned1994-08-01en_US
dc.date.accessioned2009-05-27T09:35:57Z
dc.date.available1994-08-01en_US
dc.date.available2009-05-27T09:35:57Z
dc.date.issued1994-08-01en_US
dc.description.abstractCalvin cycle multienzyme complex, consisting of phosphoriboisomerase, phosphoribulokinase and ribulose-1,5-bisphosphate carboxylase (Rubisco), shows ribose-5-phosphate + ATP dependent CO2 fixation activity with a small but discernible lag. Transient time analysis showed that the lag at pH 7 was independent of multienzyme concentration and was significantly lower than the expected transient time calculated from Km and Vmax of the individual enzymes, indicative of channeling of the intermediates in the enzyme complex. Channeling of ribulose-1,5-bisphosphate was found to offer a catalytic advantage to Rubisco. Rubisco shows a decrease in activity during catalysis in ribulose-1,5-bisphosphate dependent CO2 fixation reaction, due to the formation of the catalytic inhibitor. Such a decrease of Rubisco activity was not observed in ribose-5-phosphate + ATP dependent CO2 fixation reaction and the catalytic inhibitor was also not detected. These results suggested that the intermediates are channeled in the complex and channeling offers a catalytic facilitation to Rubisco.en_US
dc.description.affiliationMolecular Biology & Agriculture Division, Bhabha Atomic Research Centre, Trombay, Bombay.en_US
dc.identifier.citationSainis JK, Jawali N. Channeling of the intermediates and catalytic facilitation to Rubisco in a multienzyme complex of Calvin cycle enzymes. Indian Journal of Biochemistry & Biophysics. 1994 Aug; 31(4): 215-20en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27990
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAldose-Ketose Isomerasesen_US
dc.subject.meshCarbohydrate Epimerases --metabolismen_US
dc.subject.meshCatalysisen_US
dc.subject.meshMultienzyme Complexes --metabolismen_US
dc.subject.meshPhosphotransferases (Alcohol Group Acceptor) --metabolismen_US
dc.subject.meshPlant Leavesen_US
dc.subject.meshRibulose-Bisphosphate Carboxylase --metabolismen_US
dc.subject.meshSpinacia oleraceaen_US
dc.titleChanneling of the intermediates and catalytic facilitation to Rubisco in a multienzyme complex of Calvin cycle enzymes.en_US
dc.typeJournal Articleen_US
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