Rise in free intracellular calcium in HeLa cells infected with aggregative Klebsiella pneumoniae strains isolated from cases of diarrhoea.

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dc.contributor.authorPal, Aen_US
dc.contributor.authorHoque, K Men_US
dc.contributor.authorNiyogi, S Ken_US
dc.contributor.authorRamamurthy, Ten_US
dc.contributor.authorNair, G Ben_US
dc.contributor.authorBhattacharya, S Ken_US
dc.contributor.authorChakrabarti, M Ken_US
dc.date.accessioned2001-01-31en_US
dc.date.accessioned2009-05-27T07:29:00Z
dc.date.available2001-01-31en_US
dc.date.available2009-05-27T07:29:00Z
dc.date.issued2001-01-31en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Klebsiella pneumoniae strains occasionally cause diarrhoea in humans. This study was done to determine the involvement of calcium in the pathogenesis of aggregative K. pneumoniae strains. METHODS: A total of nine strains of K. pneumoniae were tested for adherence assay in HeLa cell line. A representative strain CO-1215 was used for [Ca2+]i study using Fura-2 fluorescence. RESULTS: Infection of cultured HeLa cells with aggregative K. pneumoniae strain resulted in five-fold elevation of intracellular level of free calcium ([Ca2+]i) with maximum Ca2+ influx at 3 h after bacterial infection. Chelation of extracellular Ca2+ with [ethylenebis(oxyethylenenitrile)] tetraacetic acid and suspension of cells in Ca2+ free buffer suggested that the rise of Ca2+ in aggregative K. pneumoniae infected HeLa cells was due to influx of Ca2+ from extracellular medium. INTERPRETATION & CONCLUSIONS: This study showed aggregative adherence in HeLa cells and this adherence leads to influx of extracellular Ca2+. The unrestricted passage of calcium ions across cell membranes could cause phosphorylation of proteins involved in ion transport across the membrane, which could result in secretory diarrhoea. Further work is in progress to study the enterotoxicity of these strains in an in vitro rabbit intestinal model.en_US
dc.description.affiliationNational Institute of Cholera & Enteric Diseases (ICMR), Calcutta, India.en_US
dc.identifier.citationPal A, Hoque KM, Niyogi SK, Ramamurthy T, Nair GB, Bhattacharya SK, Chakrabarti MK. Rise in free intracellular calcium in HeLa cells infected with aggregative Klebsiella pneumoniae strains isolated from cases of diarrhoea. Indian Journal of Medical Research. 2001 Jan; 113(): 1-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/20890
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshCalcium --metabolismen_US
dc.subject.meshDiarrhea --microbiologyen_US
dc.subject.meshHela Cells --metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshIntracellular Membranes --metabolismen_US
dc.subject.meshKlebsiella Infections --metabolismen_US
dc.subject.meshKlebsiella pneumoniae --isolation & purificationen_US
dc.titleRise in free intracellular calcium in HeLa cells infected with aggregative Klebsiella pneumoniae strains isolated from cases of diarrhoea.en_US
dc.typeJournal Articleen_US
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