Sensitivity and specificity of an in-house rapid urease test for detecting Helicobacter pylori infection on gastric biopsy.

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dc.contributor.authorChomvarin, Chariyaen_US
dc.contributor.authorChantarasuk, Yingriten_US
dc.contributor.authorMairiang, Pisalnen_US
dc.contributor.authorKularbkaew, Churairaten_US
dc.contributor.authorSangchan, Apichaten_US
dc.contributor.authorChanlertrith, Kittien_US
dc.contributor.authorNamwat, Wisesen_US
dc.date.accessioned2009-05-27T15:59:15Z
dc.date.available2009-05-27T15:59:15Z
dc.date.issued2006-03-28en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractWe developed an in-house rapid urease test (iRUT) and evaluated the efficacy and the agreement of the iRUT and the cRUT compared with culture and histology for the detection of H. pylori infection. Five iRUT media were tested with H. pylori isolates and other bacteria. The most suitable iRUT medium was further evaluated for detection of H. pylori infection. Gastric biopsies from 120 patients were diagnosed by culture, iRUT, cRUT and histology. The results of the iRUT and cRUT were read at 30 minutes, 1 hour and up to 24 hours. A true positive result was either the culture or both the RUT (cRUT or iRUT) and the histological examination being positive. The sensitivity and specificity of the iRUT result at 30 minutes, 1 hour and up to 24 hours were 77.1% and 100%, 77.6% and 100%, and 94.1% and 94.2%, respectively. Values for the same parameters of cRUT were 87.5% and 100%, 89.8% and 100%, and 100% and 94.2%, respectively. The agreement between the iRUT and cRUT was very good (kappa values > or = 0.82). Our results indicate that the iRUT is a-sensitive, specific and cost effective test. It can be appropriately applied for detecting H. pylori infection in gastric biopsy specimens.en_US
dc.description.affiliationDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. chariya@kku.ac.then_US
dc.identifier.citationChomvarin C, Chantarasuk Y, Mairiang P, Kularbkaew C, Sangchan A, Chanlertrith K, Namwat W. Sensitivity and specificity of an in-house rapid urease test for detecting Helicobacter pylori infection on gastric biopsy. The Southeast Asian Journal of Tropical Medicine and Public Health. 2006 Mar; 37(2): 312-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34144
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2006_37_2/12-3683.pdfen_US
dc.subject.meshBiopsyen_US
dc.subject.meshColony Count, Microbialen_US
dc.subject.meshHelicobacter Infections --diagnosisen_US
dc.subject.meshHelicobacter pylori --enzymologyen_US
dc.subject.meshHumansen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshStomach --microbiologyen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshUrease --metabolismen_US
dc.titleSensitivity and specificity of an in-house rapid urease test for detecting Helicobacter pylori infection on gastric biopsy.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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