Monoclonal antibody affinity purification of a 78 kDa membrane protein of Leishmania donovani of Indian origin and its role in host-parasite interaction.

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dc.contributor.authorMukherjee, Mandiraen_US
dc.contributor.authorBhattacharyya, Aninditaen_US
dc.contributor.authorDuttagupta, Swadeshen_US
dc.date.accessioned2002-12-07en_US
dc.date.accessioned2009-06-01T15:01:38Z
dc.date.available2002-12-07en_US
dc.date.available2009-06-01T15:01:38Z
dc.date.issued2002-12-07en_US
dc.description.abstractMonoclonal antibodies were raised against pathogenic promastigotes of Leishmania donovani of Indian origin. Among these, one was used for immuno-affinity purification of a 78 kDa membrane protein present in both the amastigote and promastigote forms of the parasite. Results of immunoblot experiments with the anti-78 kDa antibody revealed that the protein was present only in parasites belonging to the L. donovani complex. The expression of the protein was observed to be the same during different phases of growth of the promastigotes. Therefore, the 78 kDa protein is neither stage-specific nor differentially regulated. Surface iodination and subcellular fractionation of the promastigotes indicated that the protein was localized on the cell surface. The 78 kDa protein was found to inhibit the binding of promastigotes to macrophages significantly, suggesting that it may play a role in the process of infection. Thus, here we report the purification of a surface protein of L. donovani of Indian origin, which may play an important role in the process of infection.en_US
dc.description.affiliationLeishmania Division, Indian Institute of Chemical Biology, 4, Raja SC Mullick Road, Jadavpur, Kolkata 700 032, India. mandira_71@yahoo.comen_US
dc.identifier.citationMukherjee M, Bhattacharyya A, Duttagupta S. Monoclonal antibody affinity purification of a 78 kDa membrane protein of Leishmania donovani of Indian origin and its role in host-parasite interaction. Journal of Biosciences. 2002 Dec; 27(7): 665-72en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111249
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonalen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFlagella --metabolismen_US
dc.subject.meshHost-Parasite Interactionsen_US
dc.subject.meshHumansen_US
dc.subject.meshLeishmania donovani --metabolismen_US
dc.subject.meshLeishmaniasis, Visceral --metabolismen_US
dc.subject.meshMacrophages --parasitologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshPrecipitin Testsen_US
dc.subject.meshTime Factorsen_US
dc.titleMonoclonal antibody affinity purification of a 78 kDa membrane protein of Leishmania donovani of Indian origin and its role in host-parasite interaction.en_US
dc.typeJournal Articleen_US
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