Evaluation of indirect immunofluorescent antibody test for detection of IgM specific antibodies in malaria.

dc.contributor.authorRay, Ken_US
dc.contributor.authorBhattacharya, Den_US
dc.contributor.authorMukherjee, A Ken_US
dc.contributor.authorUpreti, H Ben_US
dc.contributor.authorChowdhuri, A Nen_US
dc.date.accessioned1992-06-01en_US
dc.date.accessioned2009-06-01T19:20:56Z
dc.date.available1992-06-01en_US
dc.date.available2009-06-01T19:20:56Z
dc.date.issued1992-06-01en_US
dc.description.abstractIndirect immunofluorescent antibody test using Plasmodium falciparum antigen from in vitro culture was evaluated for detecting IgM antibodies in order to determine the feasibility of its application in serodiagnosis of malaria. Test was compared with the already adapted IgG-IIF test using the same antigen. It was found that none of the healthy controls and slide negative fever cases had malaria IgM antibodies whereas 8 per cent of healthy controls and 49.01 per cent of the slide negative fever cases had malaria IgG antibodies. The sensitivity of IgM-IIF test was 94.68 per cent and that of IgG-IIF test was 96.81 per cent. IgM antibodies could be detected very early even on the first day of fever and titre rose gradually with increasing number of days of illness before institution of treatment. The IgM antibodies, being short lasting are able to reflect recent infection. The test although highly sensitive and specific is laborious and expensive. Therefore, it may be used as a serodiagnostic test in advanced laboratories only for confirmation of selected slide negative cases.en_US
dc.description.affiliationTraining & Research Centre, Safdarjung Hospital, New Delhi.en_US
dc.identifier.citationRay K, Bhattacharya D, Mukherjee AK, Upreti HB, Chowdhuri AN. Evaluation of indirect immunofluorescent antibody test for detection of IgM specific antibodies in malaria. Journal of Communicable Diseases. 1992 Jun; 24(2): 82-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/112888
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Protozoan --blooden_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshFeasibility Studiesen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --blooden_US
dc.subject.meshImmunoglobulin M --blooden_US
dc.subject.meshMalaria, Falciparum --blooden_US
dc.subject.meshMalaria, Vivax --blooden_US
dc.subject.meshPlasmodium falciparum --immunologyen_US
dc.subject.meshPlasmodium vivax --immunologyen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleEvaluation of indirect immunofluorescent antibody test for detection of IgM specific antibodies in malaria.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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