Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis.

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dc.contributor.authorWang, Yicun
dc.contributor.authorGao, Xiang
dc.contributor.authorgang, JU Zhi
dc.contributor.authorLiu, Jingyuan
dc.contributor.authorDong, Shuai
dc.contributor.authorWang, Li
dc.date.accessioned2015-02-09T09:40:11Z
dc.date.available2015-02-09T09:40:11Z
dc.date.issued2013-07
dc.description.abstractBackground: The secreted aspartyl proteinases 2 (Sap2) of Candida albicans (C. albicans) is a potential marker of candididasis. It is a virulence factor associated with adherence and tissue invasion. Aim: In order to detect Sap2 in clinical sera, we developed an indirect competitive enzyme-linked immunosorbent assay (ELISA). Materials and Methods: Polyclonal antibodies were produced for Sap2 by injecting Sap2 into a New Zealand White inbred rabbit. They could be used at a dilution exceeding 1:1200 in an indirect ELISA, and detected Sap2 concentration up to 1 ng/mL. Results: Of the 286 cancer serum samples tested, 16.8% were found as candidiasis. The test was simple and economical to perform and had a level of sensitivity for detection of low-titer positive sera; thus, it may be proven to be of value in epidemiological studies on candidiasis.en_US
dc.identifier.citationWang Yicun, Gao Xiang, gang JU Zhi, Liu Jingyuan, Dong Shuai, Wang Li. Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis. Indian Journal of Pathology & Microbiology. 2013 Jul-Sept 56 (3): 243-247.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/155876
dc.language.isoenen_US
dc.source.urihttps://www.ijpmonline.org/article.asp?issn=0377-4929;year=2013;volume=56;issue=3;spage=243;epage=247;aulast=Wangen_US
dc.subjectAntibodyen_US
dc.subjectcanceren_US
dc.subjectCandida albicansen_US
dc.subjectELISAen_US
dc.subjectSap2en_US
dc.titleDetection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis.en_US
dc.typeArticleen_US
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