Intratypic variations among Thai herpes simplex virus (HSV) isolates determined by restriction fragment length polymorphism (RFLP) analysis.
dc.contributor.author | Visaprom, Sutida | en_US |
dc.contributor.author | Chindamporn, Ariya | en_US |
dc.contributor.author | Chantratita, Wasun | en_US |
dc.contributor.author | Bhattarakosol, Parvapan | en_US |
dc.date.accessioned | 2009-05-27T16:00:17Z | |
dc.date.available | 2009-05-27T16:00:17Z | |
dc.date.issued | 2005-07-22 | en_US |
dc.description | The Southeast Asian Journal of Tropical Medicine and Public Health. | en_US |
dc.description.abstract | Whole genomic polymorphisms for 20 HSV-1 and 20 HSV-2 isolates from Thai patients were analyzed by means of Restriction Fragment Length Polymorphism (RFLP) analysis using 4 restriction endonucleases: BamHI, Kpnl, HindIII, and EcoRI. Variations in cleavage sites among the HSV-1 and HSV-2 isolates were compared to the cleavage patterns of standard HSV-1 strain KOS and HSV-2 strain Baylor 186. Although 70% of HSV-1 isolates with BamHI digestion, 50% with Kpnl, 75% with HindIII and 70% with EcoRI digestion were found to be similar to the standard HSV-1 (KOS) pattern, new BamHI restriction sites were detected in some HSV-1 isolates. For HSV-2 isolates, 85% had the same pattern as the standard HSV-2 (Baylor 186) after digestion with BamHI, HindIII, and EcoRI. No difference was observed with Kpnl digestion. When the patterns from the 4 enzymes were combined, HSV-1 isolates showed more divergence than the HSV-2 isolates. HSV-1 isolates found in both non-genital and genital lesions had more variety than the HSV-2 isolates. This suggests that intratypic variations in HSV-2 are fewer than in HSV-1. | en_US |
dc.description.affiliation | Inter-department of Medical Microbiology, Faculty of Graduate School, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. | en_US |
dc.identifier.citation | Visaprom S, Chindamporn A, Chantratita W, Bhattarakosol P. Intratypic variations among Thai herpes simplex virus (HSV) isolates determined by restriction fragment length polymorphism (RFLP) analysis. The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 Jul; 36(4): 910-6 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/34186 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.tm.mahidol.ac.th/seameo/2005_36_4/16-3487.pdf | en_US |
dc.subject.mesh | DNA, Viral | en_US |
dc.subject.mesh | Genetic Variation | en_US |
dc.subject.mesh | Herpesvirus 1, Human --genetics | en_US |
dc.subject.mesh | Herpesvirus 2, Human --genetics | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Polymerase Chain Reaction | en_US |
dc.subject.mesh | Polymorphism, Restriction Fragment Length | en_US |
dc.subject.mesh | Thailand | en_US |
dc.title | Intratypic variations among Thai herpes simplex virus (HSV) isolates determined by restriction fragment length polymorphism (RFLP) analysis. | en_US |
dc.type | Journal Article | en_US |
dc.type | Research Support, Non-U.S. Gov't | en_US |