Intratypic variations among Thai herpes simplex virus (HSV) isolates determined by restriction fragment length polymorphism (RFLP) analysis.

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dc.contributor.authorVisaprom, Sutidaen_US
dc.contributor.authorChindamporn, Ariyaen_US
dc.contributor.authorChantratita, Wasunen_US
dc.contributor.authorBhattarakosol, Parvapanen_US
dc.date.accessioned2009-05-27T16:00:17Z
dc.date.available2009-05-27T16:00:17Z
dc.date.issued2005-07-22en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractWhole genomic polymorphisms for 20 HSV-1 and 20 HSV-2 isolates from Thai patients were analyzed by means of Restriction Fragment Length Polymorphism (RFLP) analysis using 4 restriction endonucleases: BamHI, Kpnl, HindIII, and EcoRI. Variations in cleavage sites among the HSV-1 and HSV-2 isolates were compared to the cleavage patterns of standard HSV-1 strain KOS and HSV-2 strain Baylor 186. Although 70% of HSV-1 isolates with BamHI digestion, 50% with Kpnl, 75% with HindIII and 70% with EcoRI digestion were found to be similar to the standard HSV-1 (KOS) pattern, new BamHI restriction sites were detected in some HSV-1 isolates. For HSV-2 isolates, 85% had the same pattern as the standard HSV-2 (Baylor 186) after digestion with BamHI, HindIII, and EcoRI. No difference was observed with Kpnl digestion. When the patterns from the 4 enzymes were combined, HSV-1 isolates showed more divergence than the HSV-2 isolates. HSV-1 isolates found in both non-genital and genital lesions had more variety than the HSV-2 isolates. This suggests that intratypic variations in HSV-2 are fewer than in HSV-1.en_US
dc.description.affiliationInter-department of Medical Microbiology, Faculty of Graduate School, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.en_US
dc.identifier.citationVisaprom S, Chindamporn A, Chantratita W, Bhattarakosol P. Intratypic variations among Thai herpes simplex virus (HSV) isolates determined by restriction fragment length polymorphism (RFLP) analysis. The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 Jul; 36(4): 910-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34186
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2005_36_4/16-3487.pdfen_US
dc.subject.meshDNA, Viralen_US
dc.subject.meshGenetic Variationen_US
dc.subject.meshHerpesvirus 1, Human --geneticsen_US
dc.subject.meshHerpesvirus 2, Human --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshThailanden_US
dc.titleIntratypic variations among Thai herpes simplex virus (HSV) isolates determined by restriction fragment length polymorphism (RFLP) analysis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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