Mutations at embB306 codon and their association with multidrug resistant M. tuberculosis clinical isolates.

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dc.contributor.authorGupta, A
dc.contributor.authorSingh, S K
dc.contributor.authorAnupurba, S
dc.date.accessioned2015-07-13T05:29:24Z
dc.date.available2015-07-13T05:29:24Z
dc.date.issued2015-07
dc.description.abstractPurpose: The presence of embB306 mutation in ethambutol (EMB)‑susceptible (EMBs) clinical isolates questions the significance of these mutations in conferring resistance to EMB. The present study was carried out to determine the occurrence of embB306 mutation in EMB‑resistant (EMBr) and EMBs strains of M. tuberculosis. One hundred and four multidrug‑resistant tuberculosis (MDR‑TB) strains were also included to establish the relevance of excessive use of rifampicin (RIF) and isoniazid (INH) in occurrence of embB306 mutations in EMBs M. tuberculosis isolates. Materials and Methods: Deoxyribonucleic acid (DNA) from M. tuberculosis clinical strains was isolated by cetyltrimethylammonium bromide (CTAB) method. Phenotypic and genotypic drug susceptibility testing (DST) was performed on 354 M. tuberculosis isolates by using standard proportion method and multiplex‑allele‑specific polymerase chain reaction assay, respectively. Results: The overall frequency of embB306 mutations in EMBr isolates was found to be five times higher than its occurrence in EMB‑susceptible isolates (50% vs 10%). Further, the association between embB306 mutation and EMB‑resistance was observed to be statistically significant (P = 0.000). Conclusion: The embB306 is not only the main causative mutation of EMB resistance, but is a sensitive applicant marker for EMB‑resistance study.en_US
dc.identifier.citationGupta A, Singh S K, Anupurba S. Mutations at embB306 codon and their association with multidrug resistant M. tuberculosis clinical isolates. Indian Journal of Medical Microbiology. 2015 Jul-Sept; 33 (3): 387-392.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/159615
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2015;volume=33;issue=3;spage=387;epage=392;aulast=Guptaen_US
dc.subjectAdaptive mutationen_US
dc.subjectembB306en_US
dc.subjectmultidrug resistanceen_US
dc.subjecttuberculosisen_US
dc.titleMutations at embB306 codon and their association with multidrug resistant M. tuberculosis clinical isolates.en_US
dc.typeArticleen_US
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