Isolation, characterization, and validation of RP-HPLC method for the quantification of quercetin in Huberantha senjiana leaf extract

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dc.contributor.authorPandiyan, Rajakannuen_US
dc.contributor.authorIlango, Kaliappanen_US
dc.date.accessioned2020-10-16T08:56:56Z
dc.date.available2020-10-16T08:56:56Z
dc.date.issued2020-05
dc.description.abstractHuberantha senjiana (Annonaceae) – an endemic tree of Pakkamalai Reserve Forest Gingee Hills, Tamil Nadu, Indiawas subjected to preliminary phytochemical tests and high-performance liquid chromatography (HPLC) fingerprintchromatogram was established for use in future research. Subsequently, the HPLC-DAD-ESI (+) MS analysis toidentify the common biomarkers revealed the presence of Quercetin, a polyphenolic flavonol in the ethyl acetateextract of the leaves. An isocratic reversed-phase (RP)-HPLC method for the quantification of quercetin was developedwith phenomenex, RP C18 column using mobile phase [(H2O (0.1% formic acid, A); (MeOH: ACN, (40:15v/v), B),40:60 (v/v)] with a flow rate of 0.8 ml/min, detection max at 254 nm with a retention time of 7.58 minutes and wasvalidated according to the International Conference on Harmonization Q2B guidelines. Linearity was achieved withthe concentration range of 5.0–17.5 μg/ml with a R2 value of 0.996. Sensitivity was demonstrated with the limit ofdetection of 2.28 µg/ml and the limit of quantification of 6.92 µg/ml. The robustness was estimated from purposefulchanges in the composition of the mobile phase, wavelength, and flow rate and the limits were Not More Than (NMT)4%. The foliar concentration of quercetin was found to be 0.0055% w/w. The structural confirmation was done by theisolation and characterization by the divergent spectral analysis. This is the first published report on the identification,quantification, and isolation of quercetin in the leaves of H. senjiana speciesen_US
dc.identifier.affiliationsDivision of Phytochemistry and Pharmacognosy, Interdisciplinary Institute of Indian System of Medicine (IIISM), SRM Institute of Science and Technology, Kattankulathur-603 203, Chengalpattu (Dt), Tamil Nadu, India.en_US
dc.identifier.affiliationsDivision of Phytochemistry and Pharmacognosy, Interdisciplinary Institute of Indian System of Medicine (IIISM), SRM Institute of Science and Technology, Kattankulathur-603 203, Chengalpattu (Dt), Tamil Nadu, India.en_US
dc.identifier.affiliationsDepartment of Pharmaceutical Chemistry, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur-603 203, Chengalpattu (Dt), Tamil Nadu, India.en_US
dc.identifier.citationPandiyan Rajakannu, Ilango Kaliappan. Isolation, characterization, and validation of RP-HPLC method for the quantification of quercetin in Huberantha senjiana leaf extract. Journal of Applied Pharmaceutical Science. 2020 May; 2020 May: 110-118en_US
dc.identifier.issn2231-3354
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/210757
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber5en_US
dc.relation.volume10en_US
dc.source.urihttps://dx.doi.org//10.7324/JAPS.2020.10515en_US
dc.subjectHuberantha senjianaen_US
dc.subjecthigh-performance liquid chromatographyen_US
dc.subjectHPLC fingerprintsen_US
dc.subjectisolationen_US
dc.subjectquercetinen_US
dc.titleIsolation, characterization, and validation of RP-HPLC method for the quantification of quercetin in Huberantha senjiana leaf extracten_US
dc.typeJournal Articleen_US
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