Preliminary report for the application of nucleic acid sequence-based amplification in detection of human cytomegalovirus (HCMV) late pp67 mRNA for diagnosis of HCMV infection.

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dc.contributor.authorPairoj, Wen_US
dc.contributor.authorSrichunrusami, Cen_US
dc.contributor.authorChantratita, Wen_US
dc.contributor.authorBodhiphala, Pen_US
dc.contributor.authorSungkanuparph, Sen_US
dc.contributor.authorVibhagool, Aen_US
dc.date.accessioned2009-05-27T18:53:48Z
dc.date.available2009-05-27T18:53:48Z
dc.date.issued2000-03-24en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractHuman cytomegalovirus (HCMV) late pp67 mRNA expression by nucleic acid sequence-based amplification (NASBA) in patients, clinically diagnosed as possible HCMV, probable HCMV disease, and no disease, was evaluated. The RNAs were isolated from 11 whole-blood samples of 11 patients for the specific amplification of the pp67 mRNA. NASBA results were compared to results from PCR assay and serological assay. The HCMV pp67 mRNA could be found in 3 of 11 patients, whereas, HCMV-DNA PCR was positive in 6 of 11 patients. PCR assay for HCMV-DNA in plasma has proved to correlate with clinical diagnosis of HCMV infection. Only 2 patient samples of NASBA positive results coincided with HCMV-DNA PCR. However, the diagnosis of clinically relevant HCMV infection by NASBA was seen. Anti-CMV IgG titers of 1:1,600 or over 1:1,600 were found in 2 of 3 NASBA positive cases and 5 of 6 HCMV-DNA positive cases, whereas, anti-CMV IgM were all negative. These results showed the correlation of HCMV infection detected by NASBA, PCR assay and anti-CMV IgG of the titers up to 1:1,600. Additionally, a low antibody titer of the HIV patient could be diagnosed by NASBA or PCR. In conclusion, pp67 mRNA NASBA appears to be a promising diagnostic tool in analysis of HCMV infection and/or disease. Its diagnostic value should be defined in the specific group for the follow-up of immunocompromised patients, such as organ transplant recipients in future prospective studies.en_US
dc.description.affiliationVirology and Molecular Microbiology Laboratory, Department of Pathology, Bangkok, Thailand.en_US
dc.identifier.citationPairoj W, Srichunrusami C, Chantratita W, Bodhiphala P, Sungkanuparph S, Vibhagool A. Preliminary report for the application of nucleic acid sequence-based amplification in detection of human cytomegalovirus (HCMV) late pp67 mRNA for diagnosis of HCMV infection. Journal of the Medical Association of Thailand. 2000 Mar; 83 Suppl 1(): S28-34en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/40066
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshCytomegalovirus --isolation & purificationen_US
dc.subject.meshCytomegalovirus Infections --diagnosisen_US
dc.subject.meshDNA, Viral --analysisen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNucleic Acid Amplification Techniquesen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRNA, Messenger --analysisen_US
dc.subject.meshRepetitive Sequences, Nucleic Aciden_US
dc.subject.meshSensitivity and Specificityen_US
dc.titlePreliminary report for the application of nucleic acid sequence-based amplification in detection of human cytomegalovirus (HCMV) late pp67 mRNA for diagnosis of HCMV infection.en_US
dc.typeClinical Trialen_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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