Lentinula edodes (Shiitake) mushroom extract protects against hydrogen peroxide induced cytotoxicty in peripheral blood mononuclear cells.

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dc.contributor.authorKuppusamy, U R
dc.contributor.authorChong, Y L
dc.contributor.authorMahmood, A A
dc.contributor.authorIndran, M
dc.contributor.authorAbdullah, Noorlidah
dc.contributor.authorVikineswary, S
dc.date.accessioned2011-11-09T05:24:36Z
dc.date.available2011-11-09T05:24:36Z
dc.date.issued2009-04
dc.description.abstractLentinula edodes (Berk) Pegler, commonly known as Shiitake mushroom has been used as medicinal food in Asian countries, especially in China and Japan and is believed to possess strong immunomodulatory property. In the present study, the methanolic extract of the fruit bodies of L. edodes was investigated for cytoprotective effect against H2O2-induced cytotoxicity in human peripheral blood mononuclear cells (PBMCs) by measuring the activities of xanthine oxidase (XO) and glutathione peroxidase (GPx) . H2O2 at a concentration of 5 μM caused 50% inhibition of PBMCs viability. The extract improved the PBMC viability and exerted a dose-dependent protection against H2O2-induced cytotoxicity. At 100 μg/ml of extract concentration, the cell viability increased by 60% compared with the PBMCs incubated with H2O2 alone. The extract also inhibited XO activity in PBMC, while showing moderate stimulatory effect on GPx. However, in the presence of H2O2 alone, both the enzyme activities were increased significantly. The GPx activity increased, possibly in response to the increased availability of H2O2 in the cell. When the cells were pretreated with the extract and washed (to remove the extract) prior to the addition of H2O2, the GPx and XO activities as well as the cell viability were comparable to those when incubated with the extract alone. Thus, it is suggested that one of the possible mechanisms via which L. edodes methanolic extract confers protection against H2O2-induced oxidative stress in PBMC is by inhibiting the superoxide-producing XO and increasing GPx activity which could rapidly inactivate H2O2.en_US
dc.identifier.citationKuppusamy U R, Chong Y L, Mahmood A A, Indran M, Abdullah Noorlidah, Vikineswary S. Lentinula edodes (Shiitake) mushroom extract protects against hydrogen peroxide induced cytotoxicty in peripheral blood mononuclear cells. Indian Journal of Biochemistry & Biophysics. 2009 Apr; 46(2): 161-165.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135189
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/4052/1/IJBB%2046%282%29%20161-165.pdfen_US
dc.subjectPeripheral blood mononuclear cellsen_US
dc.subjectLentinus edodesen_US
dc.subjectOxidative stressen_US
dc.subjectCytotoxicityen_US
dc.subjectGlutathione peroxidaseen_US
dc.subjectXanthine oxidaseen_US
dc.subject.meshCell Survival --drug effects
dc.subject.meshCytotoxins --antagonists & inhibitors
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshGlutathione Peroxidase --metabolism
dc.subject.meshHumans
dc.subject.meshHydrogen Peroxide --antagonists & inhibitors
dc.subject.meshHydrogen Peroxide --metabolism
dc.subject.meshHydrogen Peroxide --toxicity
dc.subject.meshLeukocytes, Mononuclear --cytology
dc.subject.meshLeukocytes, Mononuclear --drug effects
dc.subject.meshLeukocytes, Mononuclear --enzymology
dc.subject.meshMethanol --chemistry
dc.subject.meshShiitake Mushrooms --chemistry
dc.subject.meshXanthine Oxidase --metabolism
dc.titleLentinula edodes (Shiitake) mushroom extract protects against hydrogen peroxide induced cytotoxicty in peripheral blood mononuclear cells.en_US
dc.typeArticleen_US
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