Cloning of ferredoxin I gene from Azotobacter vinelandii using synthetic oligonucleotide probes.
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Date
1988-09
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Abstract
Two synthetic oligonucleotide probe mixtures, whose sequences were inferred
from two separate stretches of amino acids, one closer to the carboxy terminal and the other
closer to the amino terminal, of ferredoxin I protein of Azotobacter vinelandii, were used to
select ferredoxin I gene clones from a cosmid gene library of Azotobacter vinelandii.
Restriction analysis revealed that 7 out of 10 selected clones were of the same type. All these
clones were found to hybridize with fixABCX genes of Rhizobium meliloti.
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Keywords
Ferredoxin, Azotobacter vinelandii, fix genes, nitrogenase, Fe–S cluster
Citation
Dimri G P, Roy K B, Das H K. Cloning of ferredoxin I gene from Azotobacter vinelandii using synthetic oligonucleotide probes. Journal of Biosciences. 1988 Sep; 13(3): 323-327.