Isolation & characterization of angiogenic factors from cultured cells & conditioned media.

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dc.contributor.authorKulkarni, Sen_US
dc.contributor.authorShethna, Y Ien_US
dc.date.accessioned1992-08-01en_US
dc.date.accessioned2009-05-27T08:01:07Z
dc.date.available1992-08-01en_US
dc.date.available2009-05-27T08:01:07Z
dc.date.issued1992-08-01en_US
dc.description.abstractAngiogenic factors were isolated by ion exchange chromatography from three established cell lines viz., HEp 2, HeLa and CHO, a primary culture of mouse mammary adenocarcinoma and from conditioned media of HEp2 and cultured mammary adenocarcinoma cells. The angiogenic activity was assayed by chicken chorioallantoic membrane assay. The angiogenic factor eluted at 0.5 M, exhibited lambda max at 258 +/- 1 nm, contained protein and nucleic acid. The cellular angiogenic factor showed a ratio of 1:1 for protein and nucleic acid whereas the secreted angiogenic factor had 3-5 parts of protein to 1 part of nucleic acid. The angiogenic factor from HEp2 and CHO cells did not bind to heparin-agarose. Microheterogeneity of the angiogenic factors was established by SDS-PAGE. Antiserum raised against the cellular angiogenic factor from HEp2 cells, showed a titre of 1:1600 by ELISA. The angiogenic factor was directly localized on whole cells by ELISA. Cellular as well as secreted angiogenic factors crossreacted with the antibody. Neutralizing effect of the antiserum on induction of angiogenesis was detected on chicken chorioallantoic membrane.en_US
dc.description.affiliationDepartment of Life Sciences, University of Bombay.en_US
dc.identifier.citationKulkarni S, Shethna YI. Isolation & characterization of angiogenic factors from cultured cells & conditioned media. Indian Journal of Medical Research. 1992 Aug; 96(): 236-44en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/22937
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAngiogenesis Inducing Agents --analysisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCHO Cellsen_US
dc.subject.meshCell Extracts --chemistryen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshCulture Media, Conditioned --chemistryen_US
dc.subject.meshFemaleen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleIsolation & characterization of angiogenic factors from cultured cells & conditioned media.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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