Synthesis, characterization and antibacterial potential of silver nanoparticles by Morus nigra leaf extract.
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Date
2013-10
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Abstract
The present study reports the synthesis of silver nanoparticle using Morus nigra leaf extract
were used as reducing agent for reduction of silver nitrate solution. The synthesis of silver
nanoparticles was analyzed by UV-Visible spectroscopy, Scanning Electron Microscopy. The
SEM analysis has shown that size of silver nanoparticles synthesized from leaves extract of
M.nigra was 200 nm and seems to be spherical in morphology. Morphology of chemically
synthesized silver nanoparticles is nearly spherical and of size ranges from 300-500 nm. The
average particle size analyzed from SEM analysis was observed to be 350 nm. This article has
discussed the synthesis of silver nanoparticles generated from plant extract, characterization
and antibacterial analysis. In this study the antibacterial activity was examined against six
MTCC cultures collected from IMTECH Chandigarh, Including both gram positive and gram
negative bacteria such as P.aeruginosa, S.aureus, B.subtilis, E.coli, P.flourescens and
Streptococus mutans. Out of these strains the antimicrobial activity of the silver nanoparticles
showed maximum zone of inbhition against P.flourescens (22 mm), P.aeruginosa (19 mm),
S.aureus (18 mm) and least effective against E.coli (15mm). In contrast chemically
synthesized silver nanoparticles were found most effective against S.aureus (13 mm) and
B.subtilis (12mm) and almost ineffective against Streptococcus mutans (6 mm) and
P.flourescens (4 mm). In the concluding remarks, the silver nanoparticles synthesized using
M.nigra leaves extract would be a better antimicrobial effective against various bacterial
species.
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Keywords
Biosynthesis, Silver nanoparticles, Antimicrobial activity, Leaf extract, M nigra
Citation
Kumar Anu, Kaur Kuldeep, Sharma Sarika. Synthesis, characterization and antibacterial potential of silver nanoparticles by Morus nigra leaf extract. Indian Journal of Pharmaceutical & Biological Research. 2013 Oct-Dec ; 1 (4): 16-24.