Comparison of immunofluorescence & culture for the diagnosis of respiratory syncytial virus infection.

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dc.contributor.authorJohn, Men_US
dc.contributor.authorCherian, Ten_US
dc.contributor.authorChristuraj, Sen_US
dc.contributor.authorJohn, T Jen_US
dc.date.accessioned1990-07-01en_US
dc.date.accessioned2009-05-27T06:32:35Z
dc.date.available1990-07-01en_US
dc.date.available2009-05-27T06:32:35Z
dc.date.issued1990-07-01en_US
dc.description.abstractIn 809 infants and children with acute respiratory infection, HEp-2 cells were used for the isolation of respiratory syncytial virus (RSV) and an indirect immunofluorescence technique (IIF) was used for the detection of RSV antigen in the epithelial cells of nasopharyngeal secretions. While RSV was detected in culture in only 87 subjects, IIF was positive for viral antigen in 158 subjects. In children with bronchiolitis and in those with pneumonia 57 and 19 per cent respectively, had evidence of RSV infection by culture or IIF. The frequency of virus antigen detection by IIF was above 90 per cent irrespective of the duration of symptoms before specimen collection. The frequency of virus isolation in culture was 86 per cent in children with less than 2 days duration of symptoms and 42 to 69 per cent in those with duration of symptoms of 2 days or more. However, this difference was not statistically significant. In conclusion, the IIF test was not only rapid, but also more sensitive for the detection of RSV infection than culture.en_US
dc.description.affiliationDepartment of Virology & Immunology, Christian Medical College & Hospital, Vellore.en_US
dc.identifier.citationJohn M, Cherian T, Christuraj S, John TJ. Comparison of immunofluorescence & culture for the diagnosis of respiratory syncytial virus infection. Indian Journal of Medical Research. 1990 Jul; 91(): 242-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/16751
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAntigens, Viral --analysisen_US
dc.subject.meshCell Lineen_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshNasopharynx --microbiologyen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshRespiratory Syncytial Viruses --immunologyen_US
dc.subject.meshRespirovirus Infections --diagnosisen_US
dc.titleComparison of immunofluorescence & culture for the diagnosis of respiratory syncytial virus infection.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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