Bioinformatics characterization of envelope glycoprotein from Kyasanur Forest disease virus

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dc.contributor.authorShil, Pen_US
dc.contributor.authorYadav, PDen_US
dc.contributor.authorPatil, AAen_US
dc.contributor.authorBalasubramanian, Ren_US
dc.contributor.authorMourya, DTen_US
dc.date.accessioned2020-04-10T01:37:37Z
dc.date.available2020-04-10T01:37:37Z
dc.date.issued2018-02
dc.description.abstractBackground & objectives: Kyasanur Forest disease (KFD) is a febrile illness characterized by haemorrhages and caused by KFD virus (KFDV), which belongs to the Flaviviridae family. It is reported to be an endemic disease in Shimoga district of Karnataka State, India, especially in forested and adjoining areas. Several outbreaks have been reported in newer areas, which raised queries regarding the changing nature of structural proteins if any. The objective of the study was to investigate amino acid composition and antigenic variability if any, among the envelope glycoprotein (E-proteins) from old and new strains of KFDV. Methods: Bioinformatic tools and techniques were used to predict B-cell epitopes and three-dimensional structures and to compare envelope glycoprotein (E-proteins) between the old strains of KFDV and those from emerging outbreaks till 2015. Results: The strain from recent outbreak in Thirthahalli, Karnataka State (2014), was similar to the older strain of KFDV (99.2%). Although mutations existed in strains from 2015 in Kerala KFD sequences, these did not alter the epitopes. Interpretation & conclusions: The study revealed that though mutations existed, there were no drastic changes in the structure or antigenicity of the E-proteins from recent outbreaks. Hence, no correlation could be established between the mutations and detection in new geographical areas. It seems that KFDV must be present earlier also in many States and due to availability of testing system and alertness coming into notice now.en_US
dc.identifier.affiliationsBioinformatics Group, ICMR-National Institute of Virology, Pune, Indiaen_US
dc.identifier.affiliationsKerala Unit, ICMR-National Institute of Virology, Alappuzha, Indiaen_US
dc.identifier.affiliationsICMR-National Institute of Virology, Pune, Indiaen_US
dc.identifier.citationShil P, Yadav PD, Patil AA, Balasubramanian R, Mourya DT. Bioinformatics characterization of envelope glycoprotein from Kyasanur Forest disease virus. Indian Journal of Medical Research. 2018 Feb; 147(2): 195-201en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195455
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber2en_US
dc.relation.volume147en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1445_16en_US
dc.subjectB-cell epitopesen_US
dc.subjectELISAen_US
dc.subjectenvelope glycoproteinen_US
dc.subjectKyasanur Forest diseaseen_US
dc.subjectphylogenetic analysesen_US
dc.subjectvirusen_US
dc.titleBioinformatics characterization of envelope glycoprotein from Kyasanur Forest disease virusen_US
dc.typeJournal Articleen_US
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