Application of bleach method to improve sputum smear microscopy for the diagnosis of pulmonary tuberculosis.

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dc.contributor.authorMutha, Anubhaen_US
dc.contributor.authorTiwari, Samataen_US
dc.contributor.authorKhubnani, Harishen_US
dc.contributor.authorMall, Sudhaen_US
dc.date.accessioned2005-10-22en_US
dc.date.accessioned2009-05-29T13:17:10Z
dc.date.available2005-10-22en_US
dc.date.available2009-05-29T13:17:10Z
dc.date.issued2005-10-22en_US
dc.description.abstractIn an active surveillance study, the bleach concentration method to improve sputum smear microscopy for the diagnosis of pulmonary tuberculosis was applied in chest symptomatics in Mahidpur block of Ujjain district in Madhya Pradesh, India. We purposely selected twenty villages with population of approximately 20,000 individuals. 664 sputum specimens from 297 chest symptomatics were collected. Ziehl-Neelsen staining was performed on direct sputum smears, smears made after bleach method and modified Petroff's method. Out of 297 chest symptomatics, 16 cases (5.38%) were positive by direct microscopy, 27 cases (9.09%) were positive by bleach method, 22 cases (7.40%) were positive by modified Petroff's method. The bleach method is safe, cheap, easy and sensitive. It can be applied for improved detection of Mycobacterium tuberculosis in hospitals and laboratories especially in settings where mycobacterial culture facilities are not available. The implementation of bleach method clearly improves case detection and can be a useful contribution in the National Tuberculosis Control Program.en_US
dc.description.affiliationDepartment of Microbiology, Institute of Environmental Management and Plant Sciences, Vikram University, and R. D. Gardi Medical College, Surasa, Ujjain.en_US
dc.identifier.citationMutha A, Tiwari S, Khubnani H, Mall S. Application of bleach method to improve sputum smear microscopy for the diagnosis of pulmonary tuberculosis. Indian Journal of Pathology & Microbiology. 2005 Oct; 48(4): 513-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/75488
dc.language.isoengen_US
dc.source.urihttps://www.ijpmonline.orgen_US
dc.subject.meshBacteriological Techniquesen_US
dc.subject.meshHumansen_US
dc.subject.meshIndiaen_US
dc.subject.meshMicroscopy --methodsen_US
dc.subject.meshMycobacterium tuberculosis --isolation & purificationen_US
dc.subject.meshSodium Hypochloriteen_US
dc.subject.meshSputum --microbiologyen_US
dc.subject.meshTuberculosis, Pulmonary --diagnosisen_US
dc.titleApplication of bleach method to improve sputum smear microscopy for the diagnosis of pulmonary tuberculosis.en_US
dc.typeComparative Studyen_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
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