A technique for identification of cellular proteins by western immunoblotting and immunoautoradiography of 2-D PAGE.

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dc.contributor.authorHailat, N Qen_US
dc.contributor.authorHanash, S Men_US
dc.date.accessioned1995-10-01en_US
dc.date.accessioned2009-05-27T09:47:31Z
dc.date.available1995-10-01en_US
dc.date.available2009-05-27T09:47:31Z
dc.date.issued1995-10-01en_US
dc.description.abstractA major effort of our work has been devoted to the identification, using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), of lymphoid polypeptides that are involved in lymphoid proliferation or differentiation. We have encountered problems during this effort pertaining to the exact localization of a polypeptide(s) in the silver stained gels that is recognized by western immunoblotting or proteins detected by autoradiography. In this paper, we present a method, using India ink stained/immuno-staining replica of 2-D gel nitrocellulose membrane (NCM) or India ink stained coupled with autoradiography in the case of phosphoproteins, which allows us to exactly localize the polypeptide spots detected by these methods in the silver stained 2-D PAGE. This method is expected to popularize and widen the use of 2-D PAGE technology in the investigation of cellular polypeptides.en_US
dc.description.affiliationMolecular Pathology Laboratory, Jordan University of Science & Technology, Irbid.en_US
dc.identifier.citationHailat NQ, Hanash SM. A technique for identification of cellular proteins by western immunoblotting and immunoautoradiography of 2-D PAGE. Indian Journal of Biochemistry & Biophysics. 1995 Oct; 32(5): 240-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28571
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshHumansen_US
dc.subject.meshPhosphoproteins --analysisen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleA technique for identification of cellular proteins by western immunoblotting and immunoautoradiography of 2-D PAGE.en_US
dc.typeJournal Articleen_US
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