In-vitro study of mouse peritoneal macrophage response to virulent and avirulent strains of mycobacteria.

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dc.contributor.authorNegi, Sanjay Singhen_US
dc.contributor.authorBramhne, H Gen_US
dc.contributor.authorGupta, Sunilen_US
dc.contributor.authorKumar, Gulshanen_US
dc.contributor.authorSingh, Usha Sorenen_US
dc.date.accessioned2003-09-31en_US
dc.date.accessioned2009-06-01T18:15:25Z
dc.date.available2003-09-31en_US
dc.date.available2009-06-01T18:15:25Z
dc.date.issued2003-09-31en_US
dc.description.abstractAn immunological study of pathogenesis of tuberculosis was carried out in BALB-c mice in-vitro. Peritoneal macrophages obtained from BALB-c mice were challenged with virulent (H37Rv) and avirulent (H37Ra, BCG, M. phlei) strains of mycobacteria. Activated peritoneal macrophages showed enlargement, presence of intracellular bacteria and vacuolation. These significant changes in macrophage morphology were clearly evidenced in cells infected with virulent strains of Mycobacterium tuberculosis i.e. H37Rv while being absent in cells infected with avirulent H37Ra, BCG and M. phlei. Virulent mycobacteria (H37Rv) survive the phagocytic action of macrophages by residing inside the vacuoles. The capacity of virulent and avirulent strain to stimulate TNF-alpha production from peritoneal macrophage of BALB-c mice was also examined at different time interval i.e. 1,2,4,6 and 8th day by measuring cytolytic activity of culture supernatant against murine fibroblast cell line. The pattern of highest TNF release was in case of H37Rv and least with M. phlei as measured in culture supernatant after 1,2,4,6 and 8th day.en_US
dc.description.affiliationNational Institute of Conmunicable Disease, 22-Shanmath Marg, Delhi.en_US
dc.identifier.citationNegi SS, Bramhne HG, Gupta S, Kumar G, Singh US. In-vitro study of mouse peritoneal macrophage response to virulent and avirulent strains of mycobacteria. Journal of Communicable Diseases. 2003 Sep; 35(3): 154-61en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/112092
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshMacrophages, Peritoneal --metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshMycobacterium phlei --pathogenicityen_US
dc.subject.meshMycobacterium tuberculosis --pathogenicityen_US
dc.subject.meshPhagocytosisen_US
dc.subject.meshTumor Necrosis Factor-alpha --biosynthesisen_US
dc.titleIn-vitro study of mouse peritoneal macrophage response to virulent and avirulent strains of mycobacteria.en_US
dc.typeJournal Articleen_US
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