HPLC studies in hemoglobinopathies.

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dc.contributor.authorColah, R Ben_US
dc.contributor.authorSurve, Ren_US
dc.contributor.authorSawant, Pen_US
dc.contributor.authorD'Souza, Een_US
dc.contributor.authorItalia, Ken_US
dc.contributor.authorPhanasgaonkar, Sen_US
dc.contributor.authorNadkarni, A Hen_US
dc.contributor.authorGorakshakar, A Cen_US
dc.date.accessioned2007-07-19en_US
dc.date.accessioned2009-05-30T14:49:43Z
dc.date.available2007-07-19en_US
dc.date.available2009-05-30T14:49:43Z
dc.date.issued2007-07-19en_US
dc.description28 references.en_US
dc.description.abstractAn accurate diagnosis of beta -thalassemia carriers, homozygous patients and identification of different structural hemoglobin variants is important for epidemiological studies as well as for management and prevention of the major hemoglobin disorders. There are many electrophoretic and chromatographic approaches for estimation of HbA2 and Hb F but cation exchange HPLC (CE-HPLC)using automated dedicated machines like the Variant Hb testing system have become the method of choice for these investigations. CE-HPLC also helps in the presumptive identification of many abnormal hemoglobin variants and has been useful for both neonatal screening of sickle cell disease as well as second trimester prenatal diagnosis of thalassemia by fetal blood analysis. Other applications of HPLC in hemoglobinopathies include separation of globin chains, measuring the ratio of gamma globin chains (Ggamma/Agamma) and the recently described denaturing HPLC for detecting mutations in both alpha and beta globin genes.en_US
dc.description.affiliationInstitute of Immunohaematology (ICMR), 13th Floor, New Multistoreyed Building, KEM Hospital Campus, Parel, Mumbai, India. colahrb@gmail.comen_US
dc.identifier.citationColah RB, Surve R, Sawant P, D'Souza E, Italia K, Phanasgaonkar S, Nadkarni AH, Gorakshakar AC. HPLC studies in hemoglobinopathies. Indian Journal of Pediatrics. 2007 Jul; 74(7): 657-62en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/83031
dc.language.isoengen_US
dc.source.urihttps://medind.nic.in/icb/icbai.shtmlen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshHemoglobins, Abnormal --analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshNeonatal Screeningen_US
dc.subject.meshPrenatal Diagnosisen_US
dc.subject.meshThalassemia --blooden_US
dc.titleHPLC studies in hemoglobinopathies.en_US
dc.typeJournal Articleen_US
dc.typeReviewen_US
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