Subinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosa causing inhibition of biofilm formation & reduction of virulence.

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dc.contributor.authorGupta, Parul
dc.contributor.authorChhibber, Sanjay
dc.contributor.authorHarjai, Kusum
dc.date.accessioned2016-11-23T09:13:30Z
dc.date.available2016-11-23T09:13:30Z
dc.date.issued2016-05
dc.description.abstractBackground & objectives: Biofilms formed by Pseudomonas aeruginosa lead to persistent infections. Use of antibiotics for the treatment of biofilm induced infection poses a threat towards development of resistance. Therefore, the research is directed towards exploring the property of antibiotics which may alter the virulence of an organism besides altering its growth. The aim of this study was to evaluate the role of subinhibitory concentration of ciprofloxacin (CIP) in inhibiting biofilm formation and virulence of P. aeruginosa. Methods: Antibiofilm potential of subinhibitory concentration of CIP was evaluated in terms of log reduction, biofilm forming capacity and coverslip assay. P. aeruginosa isolates (grown in the presence and absence of sub-MIC of CIP) were also evaluated for inhibition in motility, virulence factor production and quorum sensing (QS) signal production. Results: Sub-minimum inhibitory concentration (sub-MIC) of CIP significantly reduced the motility of P. aeruginosa stand and strain and clinical isolates and affected biofilm forming capacity. Production of protease, elastase, siderophore, alginate, and rhamnolipid was also significantly reduced by CIP. Interpretation & conclusions: Reduction in virulence factors and biofilm formation was due to inhibition of QS mechanism which was indicated by reduced production of QS signal molecules by P. aeruginosa in presence of subinhibitory concentration of CIP.en_US
dc.identifier.citationGupta Parul, Chhibber Sanjay, Harjai Kusum. Subinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosa causing inhibition of biofilm formation & reduction of virulence. Indian Journal of Medical Research. 2016 May; 143(5): 643-651.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/178753
dc.language.isoenen_US
dc.source.urihttps://www.icmr.nic.in/ijmr/2016/May/0515.pdfen_US
dc.subjectBiofilmen_US
dc.subjectciprofloxacinen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectquorum sensingen_US
dc.subjectvirulence factorsen_US
dc.titleSubinhibitory concentration of ciprofloxacin targets quorum sensing system of Pseudomonas aeruginosa causing inhibition of biofilm formation & reduction of virulence.en_US
dc.typeArticleen_US
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