Molecular typing of Mycobacterium tuberculosis isolates from different parts of India based on IS6110 element polymorphism using RFLP analysis.

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dc.contributor.authorChauhan, D Sen_US
dc.contributor.authorSharma, V Den_US
dc.contributor.authorParashar, Deeptien_US
dc.contributor.authorChauhan, Aradhanaen_US
dc.contributor.authorSingh, Den_US
dc.contributor.authorSingh, H Ben_US
dc.contributor.authorDas, Ren_US
dc.contributor.authorAggarwal, B Men_US
dc.contributor.authorMalhotra, Ben_US
dc.contributor.authorJain, Amitaen_US
dc.contributor.authorSharma, Meeraen_US
dc.contributor.authorKataria, V Ken_US
dc.contributor.authorAggarwal, J Ken_US
dc.contributor.authorHanif, Mohamaden_US
dc.contributor.authorShahani, Arunaen_US
dc.contributor.authorKatoch, V Men_US
dc.date.accessioned2007-04-30en_US
dc.date.accessioned2009-05-27T07:10:59Z
dc.date.available2007-04-30en_US
dc.date.available2009-05-27T07:10:59Z
dc.date.issued2007-04-30en_US
dc.description.abstractBACKGROUND & OBJECTIVE: IS 6110 based typing remains the internationally accepted standard and continues to provide new insights into the epidemiology of Mycobacterium tuberculosis. The aim of the study was to characterize M. tuberculosis isolates obtained from different parts of India based on IS6110 element polymorphism using restriction fragment length polymorphism (RFLP) analysis. METHODS: RFLP was analyzed among 308 isolates of M. tuberculosis deposited in the Mycobacterial Repository Centre, Agra, from different parts of India. DNAs isolated from these strains were restricted with Pvu II, transferred on to nylon membrane and hybridized with a PCR amplified DIG-labeled 245 bp IS6110 probe. RESULTS: Based on the copy number, M. tuberculosis isolates were classified into four groups, (i) lacking IS6110 element; (ii) low copy number (1-2); (iii) intermediate copy number (3-5); and (iv) high copy number (6-19). Copy number higher than 19 however was not observed in any of the isolates studied. At the national level, 56 per cent of the isolates showed high copy number of IS6110, 13 per cent showed intermediate copy number, 20 per cent showed low copy number, whereas 11 per cent isolates lacked IS6110 element. At the regional level, there was not much difference in the RFLP profiles of isolates (IS6110 copy numbers/patterns) from different parts of the country. INTERPRETATION & CONCLUSION: IS6110 DNA based fingerprinting could be a potentially useful tool for investigating the epidemiology of tuberculosis in India.en_US
dc.description.affiliationNational JALMA Institute for Leprosy & Other Mycobacterial Diseases, Agra, India.en_US
dc.identifier.citationChauhan DS, Sharma VD, Parashar D, Chauhan A, Singh D, Singh HB, Das R, Aggarwal BM, Malhotra B, Jain A, Sharma M, Kataria VK, Aggarwal JK, Hanif M, Shahani A, Katoch VM. Molecular typing of Mycobacterium tuberculosis isolates from different parts of India based on IS6110 element polymorphism using RFLP analysis. Indian Journal of Medical Research. 2007 Apr; 125(4): 577-81en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/19648
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshBacterial Typing Techniquesen_US
dc.subject.meshGene Dosageen_US
dc.subject.meshHumansen_US
dc.subject.meshIndia --epidemiologyen_US
dc.subject.meshMycobacterium tuberculosis --classificationen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshTuberculosis --epidemiologyen_US
dc.titleMolecular typing of Mycobacterium tuberculosis isolates from different parts of India based on IS6110 element polymorphism using RFLP analysis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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