Detection of heterogeneous, intermediate-vancomycin-resistant Staphylococcus aureus (hVISA) using low-concentration vancomycin disks.

dc.contributor.authorLulitanond, Aroonlugen_US
dc.contributor.authorChanawong, Aroonwadeeen_US
dc.contributor.authorSribenjalux, Pipaten_US
dc.contributor.authorKaewkes, Wanlopen_US
dc.contributor.authorVorachit, Malaien_US
dc.contributor.authorChongtrakool, Piriyapornen_US
dc.contributor.authorLeumsai, Danaisaken_US
dc.contributor.authorMonpou, Pensrien_US
dc.date.accessioned2009-05-27T16:12:38Z
dc.date.available2009-05-27T16:12:38Z
dc.date.issued2006-07-24en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractHeterogeneous, intermediate-vancomycin-resistant Staphylococcus aureus (hVISA) represents a threat of an incurable infection since the first report in 1997. The method used to detect hVISA isolates is a population analysis profile (PAP); however, it is impractical for routine laboratory analysis. We therefore tested a simple, reliable and inexpensive method for the detection of hVISA. Eighteen isolates of hVISA and 22 of vancomycin-sensitive S. aureus (VSSA) were included. The organisms were tested by the disk diffusion method, using 15-microg vancomycin disks on four different media: Mueller-Hinton agar (MHA), MHA plus 2% NaCI (MHAS), Brain Heart Infusion agar (BHA), and BHA plus 2% NaCl (BHAS). In addition, two different inoculum sizes, bacterial suspensions adjusted to 0.5 and 2.0 McFarland, were tested. The inhibition zone was read independently by three medical technologists after incubation at 37 degrees C for 24 and 48 hours. The use of MHAS with an inoculum size of 2.0 McFarland and 48-hour incubation period yielded the highest sensitivity (94.4%), specificity (81.8%), positive predictive value (80.9%), and negative predictive value (94.7%). The disk diffusion test with 15-microg vancomycin disk is simple and may be used as a screening method for the detection of hVISA.en_US
dc.description.affiliationFaculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. arolul@kku.ac.then_US
dc.identifier.citationLulitanond A, Chanawong A, Sribenjalux P, Kaewkes W, Vorachit M, Chongtrakool P, Leumsai D, Monpou P. Detection of heterogeneous, intermediate-vancomycin-resistant Staphylococcus aureus (hVISA) using low-concentration vancomycin disks. The Southeast Asian Journal of Tropical Medicine and Public Health. 2006 Jul; 37(4): 761-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34679
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2006_37_4/22-3773.pdfen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDisk Diffusion Antimicrobial Tests --methodsen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshStaphylococcus aureus --isolation & purificationen_US
dc.subject.meshVancomycin Resistanceen_US
dc.titleDetection of heterogeneous, intermediate-vancomycin-resistant Staphylococcus aureus (hVISA) using low-concentration vancomycin disks.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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