A single injection of 19 kDa carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 (PyMSP119) formulated with Montanide ISA and CpG ODN induces protective immune response in mice.

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dc.contributor.authorHirunpetcharat, Chakrit
dc.contributor.authorMahakunkijcharoen, Yuvadee
dc.contributor.authorJeamwattanalert, Pimmada
dc.contributor.authorKittigul, Leera
dc.contributor.authorMahannop, Pakpimol
dc.contributor.authorPichyangkul, Sathit
dc.date.accessioned2012-01-12T06:38:10Z
dc.date.available2012-01-12T06:38:10Z
dc.date.issued2011-09
dc.description.abstractObjective: To investigate the efficacy of a vaccine formulation of the 19 kDa conserved carboxyl-terminal fragment of Plasmodium yoelii merozoite surface protein-1 (PyMSP119) formulated with CpG ODN 1826 and Montanide ISA51 or ISA720 when used to immunize mice by a single injection. Methods: Groups of BALB/c mice were immunized parenterally with one, two or four injections with PBS or PyMSP119 formulated with CpG ODN in ISA51 or ISA720. Sera were collected weekly and assessed for total IgG and IgG subclass titers. Protection was tested by challenge infection with P. yoelii YM. Results: Interestingly, single injection immunization showed the same kinetics of antibody responses as two- or four-injection immunization. However, the peak antibody response induced by PyMSP119 in CpG ODN and ISA51 appeared earlier than that induced by PyMSP119 in CpG ODN and ISA720 (28 days vs 41 days). At day 63 after the first injection, the PyMSP119-specific IgG antibody levels by single injection and four- injection immunizations were not different. However, the levels of the IgG2a antibody subclass were significantly lower by single injection immunization with PyMSP119 in CpG ODN and ISA720. The antibodies were sustained at high levels for at least 20 weeks. After challenge infection, all mice immunized by a single injection of PyMSP119 in CpG ODN and ISA51 survived with low-grade parasitemia, while 50% of mice immunized with PyMSP119 in CpG ODN and ISA720 died with high levels of parasitemia. Conclusion: These findings suggest that MSP119 immunization by a single injection can induce protective immunity, particularly when formulated with an appropriate strong adjuvant.en_US
dc.identifier.citationHirunpetcharat Chakrit, Mahakunkijcharoen Yuvadee, Jeamwattanalert Pimmada, Kittigul Leera, Mahannop Pakpimol, Pichyangkul Sathit. A single injection of 19 kDa carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 (PyMSP119) formulated with Montanide ISA and CpG ODN induces protective immune response in mice. Asian Pacific Journal of Allergy and Immunology, 2011 Sept; 29(3): 252-259.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/136421
dc.language.isoenen_US
dc.source.urihttps://apjai.digitaljournals.org/index.php/apjai/article/viewFile/510/466en_US
dc.subjectimmunizationen_US
dc.subjectMSP1en_US
dc.subjectmalariaen_US
dc.subjectPlasmodium yoeliien_US
dc.titleA single injection of 19 kDa carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 (PyMSP119) formulated with Montanide ISA and CpG ODN induces protective immune response in mice.en_US
dc.typeArticleen_US
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