Typing of Plasmodium falciparum DNA from 2 years old Giemsa‑stained dried blood spots using nested polymerase chain reaction assay.

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dc.contributor.authorKumar, D
dc.contributor.authorDhiman, S
dc.contributor.authorRabha, B
dc.contributor.authorGoswami, D
dc.contributor.authorYadav, K
dc.contributor.authorVeer, V
dc.contributor.authorBaruah, I
dc.date.accessioned2016-08-10T11:12:02Z
dc.date.available2016-08-10T11:12:02Z
dc.date.issued2016-04
dc.description.abstractA panel of 129 Giemsa‑stained thick blood spots (TBS) confirmed for Plasmodium falciparum infection having different levels of parasite density were collected from a malaria endemic area. DNA was extracted and nested polymerase chain reaction (PCR) assay was performed to amplify P. falciparum DNA. Nested PCR assay successfully amplified P. falciparum DNA at a very low parasitaemia of ~10 parasites/μl of blood. Current PCR assay is very simple and can be used retrospectively to monitor the invasion and prevalence of different Plasmodium species in endemic areas.en_US
dc.identifier.citationKumar D, Dhiman S, Rabha B, Goswami D, Yadav K, Deka M, Veer V, Baruah I. Typing of Plasmodium falciparum DNA from 2 years old Giemsa‑stained dried blood spots using nested polymerase chain reaction assay. Indian Journal of Medical Microbiology. 2016 Apr-June; 34(2): 210-212.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/176590
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2016;volume=34;issue=2;spage=210;epage=212;aulast=Kumaren_US
dc.subjectParasitaemiaen_US
dc.subjectPlasmodiumen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectthick blood spotsen_US
dc.titleTyping of Plasmodium falciparum DNA from 2 years old Giemsa‑stained dried blood spots using nested polymerase chain reaction assay.en_US
dc.typeArticleen_US
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