Analysis of P53 Protein Expression in Odontogenic Cyst – An Immunohistochemical Study
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Date
2023-08
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Publisher
International Research Organization for Life & Health Sciences
Abstract
Introduction: p53 is a tumor suppressor gene which arrests the cell cycle at G1 phase. At this phase, it allows the repair of possible DNA damage and prevents the cell cycle progression to S phase. In normal cells, it is found at low level whereas its expression is elevated in many tumor. Hence, p53 is used as a marker of neoplasia, malignancy, and tumor progression. Purpose: The aim of the study was to evaluate the expression of P53 protein in biopsy specimen of Odontogenic Keratocyst (OKCs), Dentigerous cyst (DCs), and Radicular cyst (RCs) by immunohistochemistry (IHC) to know the biological behavior of these cases. Materials and Methods: This study is carried out in histopathologically diagnosed 15 cases of OKCs, 10 cases of DCs and 10 cases of RCs that were formalin fixed, processed, and paraffin embedded. Sections of 4-micron thickness were cut from paraffin embedded tissue blocks and stained with IHC marker p53 following standardized protocol. The presence of brown colored nuclear reaction at the site of target antigen was considered immune positive for p53. Results: In our study, we found in OKCs, the intensity of p53 expression was more for supra basal layers (46% in the supra basal layers showed intense positivity) than basal and superficial layers. In DCs and RCs, the more intensity of p53 expression in basal layer than supra basal and superficial layers was found. Conclusions: p53 was over expressed in OKCs compared with other lesions. Mutations in the p53 gene yield a p53 protein which has an increased half-life, thus allowing this mutated protein to be detected immunohistochemically. p53 protein is related to cell proliferation activities in OKCs.
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Keywords
Dentigerous cyst, Immunohistochemistry, Odontogenic cysts, Odontogenic Keratocyst, p53 protein, Radicular cyst
Citation
Meenakshi, Sharma G, Pabri R, Savoriya G, Vaish S, Devi M. Analysis of P53 Protein Expression in Odontogenic Cyst – An Immunohistochemical Study. International Journal of Scientific Study. 2023 Aug; 11(5): 25-30