Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue.

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dc.contributor.authorPeloi, Lilian Sen_US
dc.contributor.authorSoares, Rafael R Sen_US
dc.contributor.authorBiondo, Carlos E Gen_US
dc.contributor.authorSouza, Vagner Ren_US
dc.contributor.authorHioka, Noboruen_US
dc.contributor.authorKimura, Elzaen_US
dc.date.accessioned2008-06-07en_US
dc.date.accessioned2009-06-01T14:33:13Z
dc.date.available2008-06-07en_US
dc.date.available2009-06-01T14:33:13Z
dc.date.issued2008-06-07en_US
dc.description.abstractThe aim of this study was to propose the use of red light-emitting diode (LED) as an alternative light source for methylene blue (MB) photosensitizing effect in photodynamic therapy (PDT). Its effectiveness was tested against Staphylococcus aureus (ATCC 26923), Escherichia coli (ATCC 26922), Candida albicans (ATCC 90028) and Artemia salina. The maximum absorption of the LED lamps was at a wavelength of 663 nm, at intensities of 2,4,6 and 12 J.cm-2 for 10, 20, 30 and 60 min of exposure, respectively. Assays with and without LED exposure were carried out in plates containing MB at concentrations of 7 to 140.8 (micro) M for microorganisms and 13.35 to 668.5 (micro) M for microorganisms or microcrustaceans.The LED exposure induced more than 93.05%, 93.7% and 93.33% of growth inhibition for concentrations of 42.2 (micro)M for S.aureus (D-value=12.05 min) and 35.2 (micro)M for E.coli (D-value=11.51 min) and C.albicans (D-value=12.18 min), respectively after 20 min of exposure. LED exposure for 1 h increased the cytotoxic effect of MB against A.salina from 27% to 75%.Red LED is a promising light device for PDT that can effectively inhibit bacteria, yeast and microcrustacean growth.en_US
dc.description.affiliationChemistry Department, State University of Maringa, Parana, Brazil.en_US
dc.identifier.citationPeloi LS, Soares RR, Biondo CE, Souza VR, Hioka N, Kimura E. Photodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue. Journal of Biosciences. 2008 Jun; 33(2): 231-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110908
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshCandida albicans --drug effectsen_US
dc.subject.meshCell Division --drug effectsen_US
dc.subject.meshEscherichia coli --drug effectsen_US
dc.subject.meshLighten_US
dc.subject.meshMethylene Blue --pharmacologyen_US
dc.subject.meshPhotochemotherapyen_US
dc.subject.meshPhotosensitizing Agents --pharmacologyen_US
dc.subject.meshStaphylococcus aureus --drug effectsen_US
dc.titlePhotodynamic effect of light-emitting diode light on cell growth inhibition induced by methylene blue.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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