Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower.

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dc.contributor.authorChakrabarty, Ren_US
dc.contributor.authorViswakarma, Nen_US
dc.contributor.authorBhat, S Ren_US
dc.contributor.authorKirti, P Ben_US
dc.contributor.authorSingh, B Den_US
dc.contributor.authorChopra, V Len_US
dc.date.accessioned2002-09-17en_US
dc.date.accessioned2009-06-01T15:02:53Z
dc.date.available2002-09-17en_US
dc.date.available2009-06-01T15:02:53Z
dc.date.issued2002-09-17en_US
dc.description.abstractA number of factors that are known to influence genetic transformation were evaluated to optimize Agrobacterium-mediated transformation of hypocotyl explants of cauliflower variety Pusa Snowball K-1. The binary vector p35SGUSINT mobilized into Agrobacterium strain GV2260 was used for transformation and transient GUS expression was used as the basis for identifying the most appropriate conditions for transformation. Explant age, preculture period, bacterial strain and density were found to be critical determinants of transformation efficiency. Using the optimized protocol, the synthetic cryIA(b) gene was mobilized into cauliflower. Molecular analyses of transgenics established the integration and expression of the transgene. Insect bioassays indicated the effectiveness of the transgene against infestation by diamondback moth (Plutella xylostella) larvaeen_US
dc.description.affiliationNational Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India.en_US
dc.identifier.citationChakrabarty R, Viswakarma N, Bhat SR, Kirti PB, Singh BD, Chopra VL. Agrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower. Journal of Biosciences. 2002 Sep; 27(5): 495-502en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111264
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshBrassica --geneticsen_US
dc.subject.meshPlants, Genetically Modifieden_US
dc.subject.meshRhizobium --geneticsen_US
dc.subject.meshTransformation, Geneticen_US
dc.titleAgrobacterium-mediated transformation of cauliflower: optimization of protocol and development of Bt-transgenic cauliflower.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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