Establishment of two new cell lines from Bombyx mori (L.) (Lepidoptera: Bombycidae) & their susceptibility to baculoviruses.

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dc.contributor.authorSudeep, A Ben_US
dc.contributor.authorMishra, A Cen_US
dc.contributor.authorShouche, Y Sen_US
dc.contributor.authorPant, Uen_US
dc.contributor.authorMourya, D Ten_US
dc.date.accessioned2002-05-05en_US
dc.date.accessioned2009-05-27T06:54:40Z
dc.date.available2002-05-05en_US
dc.date.available2009-05-27T06:54:40Z
dc.date.issued2002-05-05en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Lepidopteran cell cultures and baculovirus expression vector systems are becoming popular due to their potential applications in biotechnology especially for the expression of foreign proteins. Efforts were made to develop new, indigenous, cell lines from Bombyx mori larvae and pupae. METHODS: Eight to ten B. mori larvae and 10-12 pupae were surface sterilized, dissected and ovaries were removed aseptically. Ovaries were chopped finely, washed and suspended in growth medium. When the cells formed monolayers, they were subcultured and experiments were carried out. RESULTS: Two new cell lines from larval and pupal ovaries of B. mori were established in Grace's insect tissue culture medium supplemented with 20 per cent FBS (foetal bovine serum). The larval cell line consisted predominantly of epithelial-like cells (98.31%), whereas the pupal cell line had a mixed cell population of epithelial-like (71.8%) and fibroblast-like cells (27.8%). Karyology indicated a typical lepidopteran pattern in both the cell lines and had chromosome numbers ranging from 35 to 150 and 60 to 180 for larval and pupal ovaries respectively. Four-fold increase in cell number was observed in these cell lines in 7 days. Both the cell lines were found susceptible to B. mori multiple nucleopolyhedrovirus and Autographa californica multiple nucleopolyhedrovirus, but not to Helicoverpa armigera single nucleopolyhedrovirus and Spodoptera litura multiple nucleopolyhedrovirus. INTERPRETATION & CONCLUSION: These well characterized cell lines may be of immense application in biotechnology and medicine for the production of biologically active recombinant proteins to use in vaccine studies as well as in therapeutic applications.en_US
dc.description.affiliationNational Institute of Virology (ICMR), Pune, India.en_US
dc.identifier.citationSudeep AB, Mishra AC, Shouche YS, Pant U, Mourya DT. Establishment of two new cell lines from Bombyx mori (L.) (Lepidoptera: Bombycidae) & their susceptibility to baculoviruses. Indian Journal of Medical Research. 2002 May; 115(): 189-93en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/18469
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBombyx --cytologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshFemaleen_US
dc.subject.meshNucleopolyhedrovirus --physiologyen_US
dc.subject.meshOvary --embryologyen_US
dc.titleEstablishment of two new cell lines from Bombyx mori (L.) (Lepidoptera: Bombycidae) & their susceptibility to baculoviruses.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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