Distribution and molecular characterization of Lasiodiplodia theobromae and Aceria guerreronis associated with coconut
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Date
2025-06
Journal Title
Journal ISSN
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Publisher
Triveni Enterprises
Abstract
Aim: A survey was conducted in major coconut-growing areas of Tamil Nadu in order to determine the presence of Aceria guerreronis, Lasiodiplodia theobromae and their interactive effects. The objective of the study was to ascertain the interaction, pathogenicity and cross infectivity reaction to infection. Methodology: Pathogenicity studies demonstrated the intensity of pest incidence, both individually and in combination, through morphological and molecular studies. Interestingly, interactions in a few crops were confirmed through PCR amplification. Results: The survey revealed a significant positive correlation between mite and fungus infestations and GPS location (?² = 2114.22, df = 1; P < 0.001). Molecular studies identified product sizes of 347 bp for Lasiodiplodia theobromae KKLT01 and 500 bp for Aceria guerreronis KKAG01. Cross-infectivity tests demonstrated that toddy palm and arecanut experienced the same effects as coconut, which was confirmed through PCR amplification. Interpretation: The present study shows that apart from mite infestation, the fungus also play a major role in increasing infestation and the interaction was positively correlated for cross infectivity. The results of the present study will help to restructure the existing management strategy.
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Keywords
Aceria guerreronis, Coconut, Infestation, Lasiodiplodia theobromae, Morpho-molecular variations
Citation
Sudha A, Kavitha PS, Rajesh M, Preetha G, Kavitha S. . Distribution and molecular characterization of Lasiodiplodia theobromae and Aceria guerreronis associated with coconut. Journal of Environmental Biology. 2025 Jun; 46(3): 389-399