mRNA translation during oocyte maturation plays a key role in development of primordial germ cells in Xenopus embryos.

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dc.contributor.authorZeynali, Bahmanen_US
dc.contributor.authorDixon, Keith Een_US
dc.date.accessioned2004-09-24en_US
dc.date.accessioned2009-06-01T14:31:06Z
dc.date.available2004-09-24en_US
dc.date.available2009-06-01T14:31:06Z
dc.date.issued2004-09-24en_US
dc.description.abstractIt is believed that cytoplasmic localization in the egg is necessary for development of primordial germ cells (PGCs) in Xenopus embryos. In this study, we sought to determine if translation of maternal mRNA during oocyte maturation is involved in the development of PGCs. Donor oocytes were collected from both stimulated (those who receive gonadotropin) and unstimulated females, artificially matured and fertilized using a host transfer technique. Using chloramphenicol (50 microM and 500 microM RNA), RNA translation was inhibited during oocyte maturation. Our results showed that in unstimulated embryos treated with 50 microM chloramphenicol, there was a significant reduction in the number of PGCs reaching genital ridges. In stimulated embryos, however, the number of PGCs was unchanged unless a higher concentration (500 microM) of chloramphenicol was used. From these results it is suggested that maternal mRNA translation during oocyte maturation plays a key role in development of PGCs.en_US
dc.description.affiliationDepartment of Biology, Faculty of Science, University of Tehran, Iran. bzeynali@khayam.ut.ac.iren_US
dc.identifier.citationZeynali B, Dixon KE. mRNA translation during oocyte maturation plays a key role in development of primordial germ cells in Xenopus embryos. Journal of Biosciences. 2004 Sep; 29(3): 355-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110883
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshChloramphenicol --metabolismen_US
dc.subject.meshCytoplasm --metabolismen_US
dc.subject.meshEmbryonic Structures --physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilizationen_US
dc.subject.meshGene Expression Regulation, Developmentalen_US
dc.subject.meshGerm Cells --metabolismen_US
dc.subject.meshGonadotropins --metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMothersen_US
dc.subject.meshOocytes --metabolismen_US
dc.subject.meshOogenesisen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRNA, Messenger --metabolismen_US
dc.subject.meshXenopus --embryologyen_US
dc.titlemRNA translation during oocyte maturation plays a key role in development of primordial germ cells in Xenopus embryos.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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