Interaction of allylisothiocyanate with bovine serum albumin.
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Date
1979-06
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Abstract
The interaction of allylisothiocyanate with bovine serum albumin
was monitored by fluorescence titration. The interaction was weak with an apparent
association constant of 2 × 102. The interaction was unaffected in the pH range
of 5·0 to 8·3 and by NaCl. However, the addition of dioxane upto 4% increased
the value of the association constant. N-Methyl bovine serum albumin and bovine
serum albumin with sulphydryl groups blocked had the same affinity for allylisothiocyanate
suggesting that amino and sulphydryl groups may not be involved
in the interaction. Polyacrylamide gel electrophoresis and estimation of available
lysine suggested that there were perhaps two types of groups involved in the interaction
of allylisothiocyanate with bovine serum albumin.
Description
Keywords
Serum albumin, allylisothiocyanate interaction
Citation
Murthy N V Kishore Kumar, Rao M S Narasinga. Interaction of allylisothiocyanate with bovine serum albumin. Journal of Biosciences. 1979 Jun; 1(2): 125-133.