Bioassays of Bacillus sphaericus (strain 1593) against mosquitoes of public health importance in Malaysia.

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dc.contributor.authorCheong, W Cen_US
dc.contributor.authorYap, H Hen_US
dc.date.accessioned2009-05-27T16:01:55Z
dc.date.available2009-05-27T16:01:55Z
dc.date.issued1985-03-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe pathogenicity of Bacillus sphaericus strain 1593 was tested against laboratory-reared larvae of four local species of mosquitoes of public health importance in Malaysia; Aedes aegypti, Anopheles balabacensis, Mansonia uniformis and Culex quinquefasciatus. The bacteria was shake-cultured at 28 +/- 1 degrees C for three days, using Glucose-Yeast Extract Salts medium. After which, the spores and vegetative cells were harvested and stored at 4 degrees C before use. Conditions for bioassays were mean temperature of 25 +/- 1 degrees C and relative humidity 65 +/- 5.0. Twenty third-instar larvae of each species were assayed in 90 ml of diluted spore solution. Each concentration and a control were replicated three times for each bioassay. Larval mortalities at 24 hours and 48 hours were taken and analyzed through Probit Analysis using a computer (IBM 370). LC50 values after 48 hours of exposure showed an increasing order of larval susceptibility as follows: Ae. aegypti (417.70 x 10(4)), An. balabacensis (45.84 x 10(4)), Ma. uniformis (18.23 x 10(4)) and Cx. quinquefasciatus (4.14 x 10(4) spores/ml). With the ability to kill 90% of the Cx. quinquefasciatus larvae tested with just a concentration of 10(5) spores/ml, B. sphaericus (strain 1593) has shown good potential as a biocontrol agent for this species of mosquito.en_US
dc.identifier.citationCheong WC, Yap HH. Bioassays of Bacillus sphaericus (strain 1593) against mosquitoes of public health importance in Malaysia. The Southeast Asian Journal of Tropical Medicine and Public Health. 1985 Mar; 16(1): 54-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34252
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBacillus --physiologyen_US
dc.subject.meshLarvaen_US
dc.subject.meshMalaysiaen_US
dc.subject.meshMosquito Controlen_US
dc.subject.meshPest Control, Biologicalen_US
dc.subject.meshSpores, Bacterialen_US
dc.titleBioassays of Bacillus sphaericus (strain 1593) against mosquitoes of public health importance in Malaysia.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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