Purification of vitellogenin from the plasma of Indian freshwater murrel, Channa punctatus (Bloch) by different methods: a comparative study.

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dc.contributor.authorSehgal, Nen_US
dc.contributor.authorGoswami, S Ven_US
dc.date.accessioned2001-08-29en_US
dc.date.accessioned2009-05-27T09:55:42Z
dc.date.available2001-08-29en_US
dc.date.available2009-05-27T09:55:42Z
dc.date.issued2001-08-29en_US
dc.description.abstractPlasma from estrogenized, [32P] NaH2PO4-injected murrel, Channa punctatus was collected in the presence of proteolytic inhibitors and subjected to different separation procedures singly or in combination, viz., gel filtration chromatography on Ultrogel AcA 34, ion-exchange chromatography on DEAE sephacel, or selective precipitation with dimethylformamide or with Mg2+: EDTA in order to isolate vitellogenin from other plasma proteins. The results show that chromatography on Ultrogel or DEAE sephacel yields intact vitellogenin whereas prior precipitation with DMF or with Mg2+: EDTA results in either co-precipitation of other plasma proteins or in the cleavage of phosvitin-like material from the native vitellogenin molecule.en_US
dc.description.affiliationDepartment of Zoology, University of Delhi, India.en_US
dc.identifier.citationSehgal N, Goswami SV. Purification of vitellogenin from the plasma of Indian freshwater murrel, Channa punctatus (Bloch) by different methods: a comparative study. Indian Journal of Biochemistry & Biophysics. 2001 Aug; 38(4): 263-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28975
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshFishesen_US
dc.subject.meshFresh Wateren_US
dc.subject.meshVitellogenins --chemistryen_US
dc.titlePurification of vitellogenin from the plasma of Indian freshwater murrel, Channa punctatus (Bloch) by different methods: a comparative study.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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