Phenotypic and plasmid pattern analysis of Staphylococcus epidermidis in bacterial keratitis.

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dc.contributor.authorNayak, Niranjanen_US
dc.contributor.authorSatpathy, Gitaen_US
dc.contributor.authorVajpayee, Rasik Ben_US
dc.contributor.authorMrudula, Srikantamen_US
dc.date.accessioned2007-01-27en_US
dc.date.accessioned2009-05-29T09:02:37Z
dc.date.available2007-01-27en_US
dc.date.available2009-05-29T09:02:37Z
dc.date.issued2007-01-27en_US
dc.description.abstractBACKGROUND: Staphylococcus epidermidis, a commensal of the conjunctival sac has been incriminated as the commonest etiological agent of bacterial keratitis. However, the pathogenic potential of this commensal organism is not clearly known. AIM: To determine any phenotypic, molecular markers of S. epidermidis pathogenicity in bacterial keratitis. MATERIALS AND METHODS: A total of 382 corneal ulcer isolates of S. epidermidis and 87 S. epidermidis isolates from healthy eyes (controls) were studied. Speciation, biotyping and antibiotic sensitivity testing were performed by conventional methods. Tube slime and adherence tests were carried out by recommended techniques. Plasmid analysis was conducted by a standard protocol. STATISTICAL ANALYSIS: Chi-square test was employed for calculations. RESULTS: Out of 382 corneal ulcer isolates (Pathogens) 284 (74.3%) belonged to biotypes I and II. Slime was detected in 164 (42.9%) of 382 pathogens vs. 21 (24.1%) of 87 controls (P<0.001). Sixty-five (39.6%) of 164 slime positive isolates were multidrug-resistant as compared to only 49 (22.4%) of 218 slime negative isolates (P<0.001). A significantly higher number i.e, 73.1% (120/164) of slime-producers possessed a 21 Kb plasmid in contrast to only 53.2% (116/218) of nonslime-producers (P<0.001). Presence of this plasmid had a statistical correlation of low significance with multidrug resistance (P=0.04). One hundred and seventy-two (45.0%) of 382 pathogens and 24 (27.6%) of the 87 controls were adherent to artificial surfaces (P=0.003) and the majority of the adherent organisms (99/172, 57.6%) were slime producers (P<0.001). CONCLUSIONS: Slime was associated with multidrug resistance in corneal ulcer isolates of S. epidermidis. The 21 Kb plasmid could determine virulence as it was responsible for slime production and adherence.en_US
dc.description.affiliationDepartment of Microbiology and Cornea Services, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India. niruni2000@yahoo.comen_US
dc.identifier.citationNayak N, Satpathy G, Vajpayee RB, Mrudula S. Phenotypic and plasmid pattern analysis of Staphylococcus epidermidis in bacterial keratitis. Indian Journal of Ophthalmology. 2007 Jan-Feb; 55(1): 9-13en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/71156
dc.language.isoengen_US
dc.source.urihttps://www.ijo.inen_US
dc.subject.meshCorneal Ulcer --microbiologyen_US
dc.subject.meshDrug Resistance, Multiple, Bacterialen_US
dc.subject.meshHumansen_US
dc.subject.meshKeratitis --microbiologyen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshStaphylococcal Infections --microbiologyen_US
dc.subject.meshStaphylococcus epidermidis --geneticsen_US
dc.titlePhenotypic and plasmid pattern analysis of Staphylococcus epidermidis in bacterial keratitis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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