Human cytomegalovirus-encoded miR-US4-1 promotes cell apoptosis and benefits discharge of infectious virus particles by targeting QARS.
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Date
2016-06
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Abstract
Human cytomegalovirus (HCMV) can cause congenital diseases and opportunistic infections in immunocompromised
individuals. Its functional proteins and microRNAs (miRNAs) facilitate efficient viral propagation by altering host cell
behaviour. Identification of functional target genes of miRNAs is an important step in studies on HCMV pathogenesis.
In this study, Glutaminyl-tRNA Synthetase (QARS), which could regulate signal transduction pathways for cellular
apoptosis, was identified as a direct target of hcmv-miR-US4-1. Apoptosis assay revealed that as silence of QARS by
ectopic expression of hcmv-miR-US4-1 and specific small interference RNA of QARS can promote cell apoptosis in
HCMV-infected HELF cells. Moreover, viral growth curve assays showed that hcmv-miR-US4-1 benefits the
discharge of infectious virus particles. However, silence of hcmv-miR-US4-1 by its specific inhibitor overturned
these effects. These results imply that hcmv-miR-US4-1 might have the same effects during HCMV nature infection.
In general, hcmv-miR-US4-1 may involve in promoting cell apoptosis and benefiting discharge of infectious virus
particles via down-regulation of QARS in HCMV-infected HELF cells.
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Keywords
Apoptosis, growth curve, hcmv-miR-US4-1, Human cytomegalovirus, infectious virus particles, QARS
Citation
Shao Yaozhong, Qi Ying, Huang Yujing, Liu Zhongyang, Ma Yanping, Guo Xin, Jiang Shujuan, Sun Zhengrong, Ruan Qiang. Human cytomegalovirus-encoded miR-US4-1 promotes cell apoptosis and benefits discharge of infectious virus particles by targeting QARS. Journal of Biosciences. 2016 June; 41(2): 183-192.