Microbiota of spent nuclear fuel pool water with emphasis on their biofilm forming ability on stainless steel (SS-304L)

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dc.contributor.authorKarley, Dugeshwaren_US
dc.contributor.authorShukla, Sudhir Ken_US
dc.contributor.authorRao, T Subbaen_US
dc.date.accessioned2020-11-18T10:14:10Z
dc.date.available2020-11-18T10:14:10Z
dc.date.issued2019-10
dc.description.abstractSpent nuclear fuel (SNF) pool is an essential unit of a nuclear power plant infrastructure, where radioactive fuel rods arekept for cooling and shielding, before reprocessing. This study explored the presence of bacteria in SNF pool water withemphasis on their capability to form biofilms on pool wall cladding material stainless steel (SS-304L). Bacteria wereisolated from SNF pool water and were characterized using 16S rRNA gene sequencing. The six bacterial isolates (Bacillussubtilis, Staphylococcus sps., S. arlettae, S. epidermidis, S. auricularis and Chryseobacterium gleum) can grow and formbiofilms at very low nutrient condition as well as in chronic radioactivity. The bacterial isolates formed biofilm on SS-304Land glass. However, the biofilm parameters assessed by CLSM microscopy showed that the strains preferred SS-304Lsurface for biofilm formation. On SS-304L, the maximum biomass (0.45 lm3/lm2) was formed by S. arlettae whencompared to maximum biomass (0.054 lm3/lm2) by Staphylococcus sp., on glass. Maximum biofilm thickness on SS304L was observed by Staphylococcus sp. (8.81 lm) when compared to that of S. epidermidis (4.16 lm) on the glasssurface. The biofilm formation on SS-304L surface suggests the possible risk of microbial-induced corrosion of SNF poolcladding material. This study highlights the need for mandatory monitoring of microbial biofilm formation in an extremeenvironment such as SNF pool.en_US
dc.identifier.affiliationsBiofouling & Thermal Ecology Section, Water & Steam Chemistry Division, Bhabha Atomic Research Centre Facilities, Kalpakkam 603 102, Indiaen_US
dc.identifier.affiliationsBiofouling & Thermal Ecology Section, Water & Steam Chemistry Division, Bhabha Atomic Research Centre Facilities, Kalpakkam 603 102, India Homi Bhabha National Institute, Mumbai 400 094, Indiaen_US
dc.identifier.affiliationsBiofouling & Thermal Ecology Section, Water & Steam Chemistry Division, Bhabha Atomic Research Centre Facilities, Kalpakkam 603 102, India Homi Bhabha National Institute, Mumbai 400 094, Indiaen_US
dc.identifier.citationKarley Dugeshwar, Shukla Sudhir K, Rao T Subba. Microbiota of spent nuclear fuel pool water with emphasis on their biofilm forming ability on stainless steel (SS-304L). Journal of Biosciences. 2019 Oct; 44(5): 1-9en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214171
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.issuenumber5en_US
dc.relation.volume44en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-019-9937-8en_US
dc.subjectBacteriaen_US
dc.subjectbiocorrosionen_US
dc.subjectbiofilmen_US
dc.subjecthazarden_US
dc.subjectspent nuclear fuelen_US
dc.subjectSS-304Len_US
dc.titleMicrobiota of spent nuclear fuel pool water with emphasis on their biofilm forming ability on stainless steel (SS-304L)en_US
dc.typeJournal Articleen_US
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