Nucleic acid amplification test: Bridging the gap in blood safety & re-evaluation of blood screening for cryptic transfusion-transmitted infection among Indian donors

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dc.contributor.authorDatta, Sen_US
dc.contributor.authorKhillan, Ken_US
dc.contributor.authorRanjan, Ven_US
dc.contributor.authorWattal, Cen_US
dc.date.accessioned2020-04-10T01:42:03Z
dc.date.available2020-04-10T01:42:03Z
dc.date.issued2019-03
dc.description.abstractBackground & objectives: Nucleic acid amplification test (NAT) in blood donor screening not only detects window period (WP) donors but also those with chronic occult infections which are negative by routine serological screening. This study was conducted to determine the time trend of NAT positivity and seroprevalence of transfusion-transmitted infections (TTIs) through a period of six years and evaluate the strength of NAT as a supplementary test in identifying the cryptic carriers in blood donor population. Methods: A total of 1,01,411 blood donations were screened between January 2011 and December 2016 by the ELISA and individual donor (ID) NAT Procleix Ultrio Plus Assay. Additional molecular and serological assays were done on the NAT yield samples to differentiate the type of cryptic carriers. Results: NAT yields comprised 0.05 per cent (50/101411) of the total samples tested with a yield rate of 1/2028. Hepatitis B virus (HBV) contributed to 80 per cent of the total NAT yields and the rest 20 per cent due to hepatitis C virus (HCV). Majority of HBV NAT yields (75%) were from chronic occult donors and 25 per cent were WP donors. Both HBV and HCV NAT yields had a wide range of viral count. There was no HIV NAT yield. A significant decline in the prevalence rate of TTIs through the study period of six years was observed. Interpretation & conclusions: The cryptic infections found in blood donors increase the risk of TTIs. Blood screening by both serology and NAT can reduce this threat.en_US
dc.identifier.affiliationsDepartment of Clinical Microbiology & Immunology, Sir Ganga Ram Hospital, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Blood Transfusion Medicine, Sir Ganga Ram Hospital, New Delhi, Indiaen_US
dc.identifier.citationDatta S, Khillan K, Ranjan V, Wattal C. Nucleic acid amplification test: Bridging the gap in blood safety & re-evaluation of blood screening for cryptic transfusion-transmitted infection among Indian donors. Indian Journal of Medical Research. 2019 Mar; 149(3): 389-395en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195825
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber3en_US
dc.relation.volume149en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_1340_17en_US
dc.subjectBlood donor screeningen_US
dc.subjectNATen_US
dc.subjecttransfusion-transmitted infectionsen_US
dc.titleNucleic acid amplification test: Bridging the gap in blood safety & re-evaluation of blood screening for cryptic transfusion-transmitted infection among Indian donorsen_US
dc.typeJournal Articleen_US
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