Construction of shuttle vectors for cloning in Vibrio cholerae and Escherichia coli.
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Date
1995-06
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Abstract
Starting from a naturally occurring cryptic plasmid pVC540 of Vibrio cholerae
non-OI. strain 1095, a number of plasmid vectors have been constructed for cloning genes
in Vibrio cholerae by introducing antibiotic resistance markers containing a set of unique
cloning sites. The constructs pVC810 and pVE920 have the origins of both Vibrio cholerae
and Escherichia coli replicons and are stable in both organisms in the absence of selective
pressure. These plasmids can serve as shuttle vectors between Escherichia coli and Vibrio
cholerae. The plasmid vectors reported here along with the demonstration of transformation
in Vibrio cholerae by plasmid DNA will facilitate genetic analysis of this important human
pathogen.
Description
Keywords
Vibrio cholerae, cryptic plasmid, shuttle vector, origin of replication, plasmid stability
Citation
Panda Dibyendu K, Banerjee Rajat, Das Jyotirmoy. Construction of shuttle vectors for cloning in Vibrio cholerae and Escherichia coli. Journal of Biosciences. 1995 Jun; 20(3): 367-376.