Rapid assay for detection of chloramphenicol acetyl transferase in Haemophilus influenzae.

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dc.contributor.authorSatish, Nen_US
dc.contributor.authorManoharan, Aen_US
dc.contributor.authorPai, Ren_US
dc.contributor.authorLalitha, M Ken_US
dc.date.accessioned2001-08-12en_US
dc.date.accessioned2009-05-27T06:47:34Z
dc.date.available2001-08-12en_US
dc.date.available2009-05-27T06:47:34Z
dc.date.issued2001-08-12en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Haemophilus influenzae causes a variety of life threatening infections in humans. Early detection of antimicrobial resistance is of importance in the treatment and management of infection. The modified Slack's method, a simple assay, has been evaluated in this study for the early detection of chloramphenicol resistance. METHODS: Fifty isolates of H. influenzae from invasive and non-invasive sites were included. Antimicrobial susceptibility testing was done by disc diffusion method and minimum inhibitory concentration (MIC) determination was performed for chloramphenicol only. Modified Slack's method was used to test for the production of chloramphenicol acetyl transferase (CAT). RESULTS: Invasive isolates showed higher degree of resistance to chloramphenicol (72%) compared to non-invasive ones (28%). One hundred per cent association was found between results of disc diffusion, MIC and CAT production amongst strains resistant to chloramphenicol. INTERPRETATION & CONCLUSION: The findings suggested that chloramphenicol still remains the drug of choice for treatment for non-invasive infection caused by H. influenzae. Modified Slack's method is a simple, rapid, inexpensive and reliable method for the detection of chloramphenicol resistance amongst H. influenzae.en_US
dc.description.affiliationDepartment of Microbiology, Christian Medical College & Hospital, Vellore, India.en_US
dc.identifier.citationSatish N, Manoharan A, Pai R, Lalitha MK. Rapid assay for detection of chloramphenicol acetyl transferase in Haemophilus influenzae. Indian Journal of Medical Research. 2001 Aug; 114(): 47-50en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/17930
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshChloramphenicol O-Acetyltransferase --analysisen_US
dc.subject.meshChloramphenicol Resistanceen_US
dc.subject.meshHaemophilus influenzae --chemistryen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshMiddle Ageden_US
dc.titleRapid assay for detection of chloramphenicol acetyl transferase in Haemophilus influenzae.en_US
dc.typeJournal Articleen_US
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