A duplicated coxI gene is associated with cytoplasmic male sterility in an alloplasmic Brassica juncea line derived from somatic hybridization with Diplotaxis catholica.

Simple item page
dc.contributor.authorPathania, Arunaen_US
dc.contributor.authorKumar, Rajeshen_US
dc.contributor.authorKumar, V Dineshen_US
dc.contributor.authorAshutosh,en_US
dc.contributor.authorDwivedi, K Ken_US
dc.contributor.authorKirti, P Ben_US
dc.contributor.authorPrakash, Sen_US
dc.contributor.authorChopra, V Len_US
dc.contributor.authorBhat, S Ren_US
dc.date.accessioned2007-08-31en_US
dc.date.accessioned2009-06-02T07:14:27Z
dc.date.available2007-08-31en_US
dc.date.available2009-06-02T07:14:27Z
dc.date.issued2007-08-31en_US
dc.description.abstractA cytoplasmic male sterile (CMS) line of Brassica juncea was derived by repeated backcrossing of the somatic hybrid (Diplotaxis catholica + B. juncea) to B. juncea. The new CMS line is comparable to euplasmic lines for almost all characters, except for flowers which bear slender, needle-like anthers with aborted pollen. Detailed Southern analysis revealed two copies of coxI gene in the CMS line. One copy, coxI-1 is similar to the coxI gene of B. juncea, whereas the second copy, coxI-2 is present in a novel rearranged region. Northern analysis with eight mitochondrial gene probes showed altered transcript pattern only for the coxI gene. Two transcripts of 2.0 and 2.4 kb, respectively, were detected in the CMS line. The novel 2.4 kb transcript was present in floral bud tissue but absent in the leaf tissue. In plants where male sterility broke down under high temperature during the later part of the growing season, the 2.4 kb coxI transcript was absent, which suggested its association with the CMS. The two coxI genes from the CMS line showed two amino acid changes in the coding region. The novel coxI gene showed unique repeats in the 5' region suggesting recombination of mitochondrial genomes of the two species. The possible role of the duplicated coxI gene in causing male sterility is discussed.en_US
dc.description.affiliationNational Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India.en_US
dc.identifier.citationPathania A, Kumar R, Kumar VD, Ashutosh , Dwivedi KK, Kirti PB, Prakash S, Chopra VL, Bhat SR. A duplicated coxI gene is associated with cytoplasmic male sterility in an alloplasmic Brassica juncea line derived from somatic hybridization with Diplotaxis catholica. Journal of Genetics. 2007 Aug; 86(2): 93-101en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/114314
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jgenet/index.htmlen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBrassica --geneticsen_US
dc.subject.meshCyclooxygenase 1 --geneticsen_US
dc.subject.meshCytoplasm --geneticsen_US
dc.subject.meshDNA, Mitochondrial --analysisen_US
dc.subject.meshFlowers --geneticsen_US
dc.subject.meshGene Duplicationen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGenome, Planten_US
dc.subject.meshHybrid Cells --metabolismen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMustard Plant --geneticsen_US
dc.subject.meshPlant Infertility --geneticsen_US
dc.subject.meshRNA --analysisen_US
dc.subject.meshRandom Amplified Polymorphic DNA Techniqueen_US
dc.subject.meshSequence Homology, Nucleic Aciden_US
dc.titleA duplicated coxI gene is associated with cytoplasmic male sterility in an alloplasmic Brassica juncea line derived from somatic hybridization with Diplotaxis catholica.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Journal of Genetics