Changes of phenolic compounds in LebZIP2-overexpressing transgenic plants

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dc.contributor.authorSeong, Eun Sooen_US
dc.contributor.authorLee, Jae Geunen_US
dc.contributor.authorChung, Ill-Minen_US
dc.contributor.authorYu, Chang Yeonen_US
dc.date.accessioned2020-01-02T06:23:24Z
dc.date.available2020-01-02T06:23:24Z
dc.date.issued2019-12
dc.description.abstractThe bZIP gene is a transcription factor that plays various roles in relation to plant stress and hormone signaling. This gene is also involved in plant environmental stress and herbicide tolerance. We generated Nicotiana benthamiana transgenic plants with LebZIP2-encoding gene isolated from tomatoes using Agrobacterium-mediated transformation. Transgenic seeds harvested from these T0 transgenic plants were grown and examined for gene transfer and changes in phenolic compounds in the T1 generation. RT-PCR analysis using a primer specific to the LebZIP gene confirmed that the gene was transferred to the T1 generation. We analyzed the increase and decrease tendency for 30 phenolic compounds using the T1 generation-transgenic plants and investigated the mechanism between the specifically increased compound and LebZIP2 gene. Gallic acid, homogentisic acid, protocatechuic acid, myricetin, t-cinnamic acid, and b-resorcyclic acid were identified as the phenolic compounds that increased in T1 transgenic plants overexpressing the LebZIP gene. Among these, homogentisic acid at 246.75-1055.19 µg/g, was increased by 2-5 fold in the T1 transgenic plants compared to the control. Protocatechuic acid was found at 1640.54-2456.00 µg/g and was increased by 2-4 fold in T1 transgenic plants. t-Cinnamic acid was present in a small amount of 23.14 µg/g in the control, whereas it was 102.19-135.47 µg/g in T1 transgenic plants, showing an increase of 4-5 folds. These results indicated that homogentisic acid, protocatechuic acid, and t-cinnamic acid among the 30 phenolic compounds analyzed, were significantly increased in LebZIP2-overexpressing T1 transgenic plants, and support the evidence that the LebZIP2 gene is significantly involved in the increment of three phenolic compounds.en_US
dc.identifier.affiliationsDepartment of Medicinal Plant, Suwon Women’s University, Suwon- 16632, Republic of Koreaen_US
dc.identifier.affiliationsHwajin Cosmetics, Hongcheon- 25142, Republic of Koreaen_US
dc.identifier.affiliationsDepartment of Applied Bioscience, Konkun University, Seoul- 143 701, Republic of Koreaen_US
dc.identifier.affiliationsDepartment of Bio-Resource Sciences, Kangwon National University, Chuncheon- 24341, Republic of Koreaen_US
dc.identifier.citationSeong Eun Soo, Lee Jae Geun, Chung Ill-Min, Yu Chang Yeon. Changes of phenolic compounds in LebZIP2-overexpressing transgenic plants. Indian Journal of Biochemistry and Biophysics. 2019 Dec; 56(6): 484-491en_US
dc.identifier.issn0975-0959
dc.identifier.issn0301-1208
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/190081
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber6en_US
dc.relation.volume56en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/52166en_US
dc.titleChanges of phenolic compounds in LebZIP2-overexpressing transgenic plantsen_US
dc.typeJournal Articleen_US
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