Role of insertion sequence element is256 as a virulence marker and its association with biofilm formation among methicillin-resistant Staphylococcus epidermidis from hospital and community settings in Chennai, South India

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dc.contributor.authorMurugesan, Sen_US
dc.contributor.authorMani, Sen_US
dc.contributor.authorKuppusamy, Ien_US
dc.contributor.authorKrishnan, Pen_US
dc.date.accessioned2020-04-23T07:35:49Z
dc.date.available2020-04-23T07:35:49Z
dc.date.issued2018-03
dc.description.abstractThe objective of this study was to detect the association of biofilm formation with IS256 among clinical and carrier isolates of methicillin-resistant Staphylococcus epidermidis (MRSE). A total of 71 MRSE isolates were included in this study. Phenotypic detection of biofilm formation was done by Congo red agar method. Detection of genes associated with biofilm formation (icaAD, aap and atlE) and insertion sequence IS256 was done by polymerase chain reaction. Of the 71 MRSE isolates,19/40 (47.5%) clinical isolates from hospital settings and 11/31 (35.5%) carrier isolates from community settings respectively were found to be positive for all the three genes tested, namely, icaAD+, aap+ and atlE+ genes. Nearly 80% of clinical isolates were found to harbour IS256, whereas only 13% of community isolates harboured IS256.en_US
dc.identifier.affiliationsDepartment of Microbiology, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai, Tamil Nadu, Indiaen_US
dc.identifier.citationMurugesan S, Mani S, Kuppusamy I, Krishnan P. Role of insertion sequence element is256 as a virulence marker and its association with biofilm formation among methicillin-resistant Staphylococcus epidermidis from hospital and community settings in Chennai, South India. Indian Journal of Medical Microbiology. 2018 Mar; 36(1): 124-126en_US
dc.identifier.issn0255-0857
dc.identifier.issn1998-3646
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/198738
dc.languageenen_US
dc.publisherIndian Association of Medical Microbiologistsen_US
dc.relation.issuenumber1en_US
dc.relation.volume36en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmm.IJMM_17_276en_US
dc.subjectIS256en_US
dc.subjectmethicillin-resistant Staphylococcus epidermidisen_US
dc.subjectmultiplex polymerase chain reactionen_US
dc.subjectvirulence determinantsen_US
dc.titleRole of insertion sequence element is256 as a virulence marker and its association with biofilm formation among methicillin-resistant Staphylococcus epidermidis from hospital and community settings in Chennai, South Indiaen_US
dc.typeJournal Articleen_US
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